【论文资料】载体通用引物序列

【论文资料】载体通用引物序列 引物名称 引物序列 3’AOX 5'-GGC AAA TGG CAT TC TGA CAT-3' 3'AD 5'-AGA TGG TGC ACG ATG CAC AG-3' 3'BD 5'-TAA GAG TCA CTT TAA AAT TTG TAT C-3' 5’AOX1 5'-GAC TGG TTC CAA TTG ACA AGC-3' A-FACTOR 5'-TAC TAT TGC CAG CAT TGC TGC-3' BAC1 5'-AAC CAT CTC GCA AAT AAA TA-3' BAC2 5'-ACG CAC AGA ATC TAG CGC TT-3' BGH rev 5'-TAG AAG GCA CAG TCG AGG C-3' CMV-F 5'-CGC AAA TGG GCG GTA GGC GTG-3' CMV-R 5'-GTT CAC GGT GCC CTC C-3' DsRed1-C-r 5'-AGC TGG ACA TCA CCT CCC ACA ACG-3' DsRed1-N-f 5'-GTA CTG GAA CTG GGG GGA CAG-3' EBV Reverse 5'-TGT GGT TTG TCC AAA CTC AAT C-3' EBV-R 5'-GTG GTT TGT CCA AAC TCA TC-3' GAL4 AD-F 5'-TAC CAC TAC AAT GGA TG-3' GAL4 BD 5'-TCA TCG GAA GAG AGT AG-3' GLP1 5'-TGT ATC TTA TGG TAC TGT AAC TG-3' GLP2 5'-CTT TAT GTT TTT GGC GTC TTC CA-3' M13(-96) 5'-CCC TCA TAG TTA GCG TAA CG-3' M13F 5'-TGT AAA ACG ACG GCC AGT-3' M13F(-47) 5'-CGC CAG GGT TTTC CCA GTC ACG AC-3' M13R 5'-CAG GAA ACA GCT ATG ACC-3' M13R(-48) 5'-AGC GGA TAA CAA TTT CAC ACA GGA-3' P24990 5'-TCATCGGAAGCGAGTAG-3' P31430 5'-CGT TTT AAAACCTAAGAGTCAC-3' P12584 5'-TTT TCA GTA TCT ACG AT-3’ P17110 5'-TAC CAC TAC AAT GGA TG-3’ PAS2-1F 5'-TCA TCG GAA GCG AGT AG-3' PAS2-1R 5'-CGT TTT AAA ACC TAA GAG TCA C-3' PB42ADF 5'-CCA GCC TCT TGC TGA GTG GAG ATG-3' PB42ADR 5'-AAG CCG ACA ACC TTG ATT GGA G-3' pbd-gal4-f 5'-GCC TCT AAC ATT GAG ACA GC-3' pbd-gal4-r 5'-AAG AGT TAC TCA AGA ACA AGA A-3' pbi-121(35s) 5'-GAC GCA CAA TCC CAC TAT CC-3' PBV220F 5'-AAG AAG GGC AGC ATT CAA AG-3' PBV220R 5'-CTG CGT TCT GAT TTA ATC TG-3' PCDNA3.0-F2 5'-CTC TGG CTA ACT AGA GAA CC-3' PCDNA3.0-R2 5'-GGC AAC TAG AAG GCA CAG TC-3' PCDNA3.1F 5'-CTA GAG AAC CCA CTG CTT AC-3’ PCDNA3.1R 5'-TAG AAG GCA CAG TCG AGG-3’ pCEP Frorward 5'-AGC AGA GCT CGT TTA GTG AAC CG-3' PCEP-F 5'-AGA GCT CGT TTA GTG AAC CG-3' PCMV-F 5'-TCT AAA AGC TGC GGA ATT GT-3' PCMV-F 5'-TTC CAA AAT GTC GTA ATA AC-3' PCMV-R 5'-TCC AAA CTC ATC AAT GTA TC-3' PCMV-R 5'-ATT ATA GAG GAC ACC TAG TC-3' PDONR-F 5'-TCG CGT TAA CGC TAG CAT GGA TCT C-3' PDONR-R 5'-GTA ACA TCA GAG ATT TTG AGA CAC-3’ PEF-F 5'-TCA AGC CTC AGA CAG TGG TTC-3' PEGFP-C-3’ 5'-TAT GGC TGA TTA TGA TCA GT-3' PEGFP-C-5’ 5'-CAT GGT CCT GCT GGA GTT CGT G-3' PEGFP-N-3’ 5'-CGT CGC CGT CCA GCT CGA CCA G-3' PEGFP-N-5’ 5'-TGG GAG GTC TAT ATA AGC AGA G-3' pETup 5'-ACG ATG CGT CCG GCG TAG AGG A-3' PGEX-3’ 5'-CCG GGA GCT GCA TGT GTC AGA GG-3' PGEX-5’ 5'-GGG CTG GCA AGC CAC GTT TGG TG-3' PIRES2-EGFP-F 5'-GTA GGC GTG TAC GGT GGG AG-3' PIRES3-EGFP-R 5'-AAC GCA CAC CGG CCT TAT TC-3' pMAL-C2X-F 5'-TGC GTA CTG CGG TGA TCA AC-3’ pMAL-C2X-R 5'-CTG CAA GGC GAT TAA GTT GG-3’ PQE30F 5'-TGA GCG GAT AAC AAT TTC AC-3' PQE30R 5'-GTT CTG AGG TCA TTA CTG G-3' pTARGET-SEQ 5'-TTA CGC CAA GTT ATT TAG GTG ACA-3’ PTRC99C-F 5'-TTG CGC CGA CAT CAT AAC-3’ PTRC99C-R 5'-CTG CGT TCT GAT TTA ATC TG-3’ PTRCHISA-F 5'-CAT GGT ATG GCT AGC ATG AC-3’ PTRCHISA-R 5'-CAG GCT GAA AAT CTT CTC TC-3’ RVP3 5'-CTA GCA AAA TAG GCT GTC CC-3' RVP4 5'-GAC GAT AGT CAT GCC CCG CG-3' S.TAG 5'-CGA ACG CCA GCA CAT GGA CA-3' S1 5'-CAA CGT GAA AAA ATT ATT ATT CGC-3’ S6 5'-GTA AAT GAA TTT TCT GTA TGA GG-3’ SP6 5'-ATT TAG GTG ACA CTA TAG-3' T3 5'-ATT AAC CCT CAC TAA AGG GA-3' T7 5'-TAA TAC GAC TCA CTA TAG GG-3' T7(17BASE) 5'-ACA TCC ACT TTG CCT TTC TC-3' T7TER 5'-TGC TAG TTA TTG CTC AGC GG-3' TRXF 5'-CAT ATG AGC GAT AAA ATT ATT CAC-3' TRXR 5'-GGA TCC CTT GTC ATC GTC ATC ACC A-3' malEfor 5'-GGT CGT CAG ACT GTC GAT GAA GCC-3' pTrcHis-fw 5'-TTA AAG AGG TAT ATA TTA ATG TAT CG-3' Xpressfor 5'-AGC ATG ACT GGT GGA CAG-3' pTrcHis-rv 5'-ATC TGT ATC AGG CTG AAA ATC-3' N-CMV-30 5'-ATA ACC CCG CCC CGT TG-3' C-CMV-24 5'-TTA GGA CAA GGC TGG TGG-3' CMV-Profor 5'-ATG GGC GGT AGG CGT G-3' pCAMBIA1301-5'(ECORI) 5'-CCA GGC TTT ACA CTT TAT GC-3' pCAMBIA1301-3'(HindIII) 5'-GCG ATT AAG TTG GGT AAC GC-3' T7 EEV 5'-ATG TCG TAA TAA CCC CGC CCC G-3' pDBLeu-5' 5'-GAA TAA GTG CGA CAT CAT CAT C- 3’ pDBLeu-3' 5'-CAG ATG GAA CGG TCT TTA AAT G-3’ HGHrev 5'-CTG GGG AGG GGT CAC AGG G-3' PSG5(BAMHI)-F 5'-GCT GGT TAT TGT GCT GTC TC-3' PSG5-R(SV40) 5'-AAA CCA CAA CTA GAA TGC AGT-3' M13R(-26) 5'-CAG GAA ACA GCT ATG AC-3' pBRforBam 5'-CTT GGA GCC ACT ATC GAC-3' pBRrevBam 5'-GGT GAT GTC GGC GAT ATA GG-3' pBRforEco 5'-AAT AGG CGT ATC ACG AGG C-3' pBRrevHind 5'-GCG TTA GCA ATT TAA CTG TG-3' c-mycrev 5'-CTC TTC TGA GAT GAG TTT TTG-3' pGAL4-ADrv 5'-GAA CTT GCG GGG TTT TTC-3' pGAL4-BDrv 5'-AAT CAT AAG AAA TTC GCC C-3' pMD19T490R 5'-CACAGGAAACAGCTATGACC-3' Psos5' 5'-CCA AGA CCA GGT ACC ATG-3' Psos3' 5'-GCC AGG GTT TTC CCA GT-3' Primer TriplEx-LD-5? 5'-CTC GGG AAG CGC GCC ATT GTG TTG G-3' Primer TriplEx-LDrv-3' 5'-ATA CGA CTC ACT ATA GGG CGA ATT GGC-3' UB-F 5'-TCA GTG TTA GAC TAG TAA ATT-3' 27F 5'-AGA GTT TGA TCC TGG CTC AG-3’ 1492R 5'-GGY TAC CTT GTT ACG ACT T-3’ PTR5 5'-CTC CGA GAT CTG GAC GAG C-3' pLXSN-F CTT GAA CCT CCT CGT TCG AC pLXSN-R GTT GCT GAC TAA TTG AGA TG pLEXA-F CGT CAG CAG AGC TTC ACC AT pLEXA-R TAA AAC CTA AGA GTC ACT TT pLNCX-F AGC TCG TTT AGT GAA CCG TCA GAT CG pLNCX-R ACC TAC AGG TGG GGT CTT TCA TTC CC pDSRED-N-R TGA AGC GCA TGA ACT CCT TG mtDNA-D-LoopF AGG ACT Acg gct tga aaa gc mtDNA-D-LoopR atg tgc ctg acc gag gaa cca g H1primer CGCTATGTGTTCTGGGAAAT pRECEIVER-M03F 5'-GCGGTAGGCGTGTACGGT-3' pRECEIVER-M03R 5'-CCGGACACGCTGAACTTGT-3' ACYCDuetUP1Primer #71178-3 GGATCTCGACGCTCTCCCT DuetUP2 Primer TTGTACACGGCCGCATAATC DuetDOWN1 GATTATGCGGCCGTGTACAA 1525R 5'-AgAAAggAggTgATCcAgcc-3' pBAD_F ATGCCATAGCATTTTTATCC pBAD_R GATTTAATCTGTATCAGG AUG1F 5'-CAATTTACATCTTTATTTATTAACG-3' AUG1R 5'-GAAGAGAAAAACATTAGTTGGC-3' V5rev 5'-ATC GAG ACC GAG GAG AGG -3' EGFP-Nrev 5'-CGT CGC CGT CCA GCT C-3' TKPAreverse 5?-CTTCCGTGTTTCAGTTAGC-3? pDsRED-express-C1-F TCC CAC AAC GAG GAC TAC AC DEST22F 5’-tataacgcgtttggaatcact-3’ DEST22R 5’-agccgacaaccttgattggagac-3’ pBMN_5' gcttggatacacgccgc pYes2.R TCG GTT AGA GCG GAT GTG pSG5-3 GAAATTTGTGATGCTATTGC pMSCV-5' CCCTTGAACCTCCTCGTTCGACC pMSCV-3' GAGACGTGCTACTTCCATTTGTC 内参通用引物 16SDNAs primer sequence 27F 5’-AGAGTTTGATCCTGGCTCAG-3’ 515F 5’-GTGCCAGCMGCCGCGGTAA-3’ 21F 5’-TTCCGGTTGATCCTGCCGGA-3’ 312F 5’-TCCTACGGGAGGCAGCAGCT-3 1492R 5’-GGYTACCTTGTTACGACTT-3’ 1525R 5'-AgAAAggAggTgATCcAgcc-3' ITS1 TCCGTAGGTGAACCTGCGG ITS4 TCCTCCGCTTATTGATATGC 真菌通用引物, nu-SSU-0817-5’ TTAGCATGGAATAATRRAATAGGA nu-SSU-1536-3’ ATTGCAATGCYCTATCCCCA