BSA的性质、作用及应用（1%、0.1%BSA是多少mg/ml）Bovineserumalbumin(BSA)makesupapproximately60%ofallproteinsinanimalserum.BSA的基本性质Numberofresidues：607Molecularweight：69323.4DapIinwaterat25℃：4.7Opticalabsorbanceof1g/lat279nm：0.667mLmg-1cm-1at279nmDimensions：40x40x140cubicangstromsA18-residuesignalpeptideiscutofffromtheprecursorproteinuponsecretion,hencetheprecursorhas607aminoacidresiduesandamolecularweightof69.4kDa.不同浓度单位的换算BSA溶液常用的浓度单位是质量体积百分比（g/ml）如：1%、0.1%；和质量/体积比如10mg/ml。这两种单位的换算如下：1%BSA=10mg/mlBSA；0.1%BSA=1mg/mlBSA根据BSA的分子量为69KDa可以估算出：10mg/mlBSA=0.145mM=145μMBSA的应用1、细胞培养时作为添加剂Itiscommonlyusedincellcultureprotocols,particularlywhenproteinsupplementationisnecessaryandtheothercomponentsofserumareunwanted.Incellcultureitsmainroleisasacarrierofsmallmolecules.Becauseofitsnegativecharge,BSAbindswater,salts,fattyacids,vitaminsandhormones,thencarriestheseboundcomponentsbetweentissuesandcells.ThebindingcapacityalsomakesBSAaneffectivescavengerforremovingtoxicsubstances,includingpyrogens,fromthemedium.2、免疫组化实验中作为封闭剂eg.ELISAs(Enzyme-LinkedImmunosorbentAssay),immunoblots,andimmunohistochemistry3、作为其他酶的保护剂eg.Inrestrictiondigests,BSAisusedtostabilizesomeenzymesduringdigestionofDNAandtopreventadhesionoftheenzymetoreactiontubesandothervessels.4、蛋白定量时作为
标准
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物eg.BradfordAssay,BCAassay5、一些酶活测定反应中作为阴性对照eg.DNAbindingassay
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