大鼠血栓素B2(TXB2)酶联免疫检测试剂盒使用说明书南建96T血清资料1

大鼠血栓素B2(TXB2)酶联免疫检测试剂盒使用说明 书 关于书的成语关于读书的排比句社区图书漂流公约怎么写关于读书的小报汉书pdf 种属适用 样本 保单样本pdf木马病毒样本下载上虞风机样本下载直线导轨样本下载电脑病毒样本下载 保存条件大鼠血清(浆)2-8℃仅用于科研，不得用于医学诊断使用前仔细阅读本说明书。本酶联免疫试剂盒是基于生物素双抗体夹心技术原理，来检测大鼠血栓素B2(TXB2),仅用于科研，不得用于医学诊断。一、用途：用于大鼠血清、血浆及相关液体样本中血栓素B2(TXB2)的测定。二、工作原理本试剂盒采用的是生物素双抗体夹心酶联免疫吸附法(ELISA)测定样品中血栓素B2(TXB2)的水平。向预先包被了大鼠血栓素82(T乂82)单克隆抗体的酶标孔中加入血栓素82(T乂82)，温育；温育后加入生物素标记的抗TXB2抗体，再与链霉亲和素-HRP结合，形成免疫复合物，再经过温育和洗涤，去除未结合的酶，然后加入底物A、B，产生蓝色，并在酸的作用下转化成最终的黄色。颜色深浅与样品中血栓素B2(TXB2)的浓度呈正相关。三、试剂盒组成编号产品名称48T96T1标准品(1280pg/ml)0.5ml0.5ml2标准品稀释液3ml3ml3酶标包被板12孔x4条12孔x8条4链霉亲和素-HRP3ml6ml5浓缩洗涤液20x20ml30x20ml6生物素标记的抗-TXB2抗体1ml1ml7显色剂A液3ml6ml8显色剂B液3ml6ml9终止液3ml6ml10说明书1份1份11封板膜2张(48T)2张(96T)12密封袋1个1个四、需要而未提供的试剂和器材1、37℃恒温箱2、标准规格酶标仪3、精密移液器及一次性吸头4、蒸馏水5、一次性试管6、吸水纸五、注意事项1、从2-8℃取出的试剂盒，在开启试剂盒之前要室温平衡至少30分钟。酶标包被板开封后如未用完，板条应装入密封袋中保存。2、各步加样均应使用加样器，并经常校对其准确性，以避免试验误差。3、严格按照说明书的操作进行，试验结果判定必须以酶标仪读数为准。4、为避免交叉污染，要避免重复使用手中的吸头和封板膜。5、不用的其它试剂应包装好或盖好。不同批号的试剂不要混用。保质期之前使用。6、底物B对光敏感，避免长时间暴露于光下。六、洗板方法手工洗板方法：甩掉酶标板内的液体；在实验台上铺垫几层吸水纸，酶标板朝下用力拍几次；将稀释后的洗涤液至少0.35ml注入孔内，浸泡1-2分钟。根据需要，重复此过程数次。自动洗板：如果有自动洗板机，应在熟练使用后再用到正式实验过程中。七、标本 要求 对教师党员的评价套管和固井爆破片与爆破装置仓库管理基本要求三甲医院都需要复审吗 1、不能检测含NaN3的样品，因NaN3抑制辣根过氧化物酶的（HRP）活性。2、标本采集后尽早进行提取，提取按相关文献进行，提取后应尽快进行实验。若不能马上进行试验，可将标本放于-20℃保存，但应避免反复冻融。八、操作程序1、标准品的稀释：（本试剂盒提供原倍标准品一支，用户请按照说明自行在小试管中倍比稀释）:640pg/ml（5号标准品）120出的原倍标准品加入120出的标准品稀释液320pg/ml（4号标准品）120出的5号标准品加入120出的标准品稀释液160pg/ml（3号标准品）120出的4号标准品加入120出的标准品稀释液80pg/ml（2号标准品）120出的3号标准品加入120出的标准品稀释液40pg/ml（1号标准品）120出的2号标准品加入120出的标准品稀释液2、根据待测样品数量加上标准品的数量决定所需的板条数。每个标准品和空白孔建议做复孔。每个样品根据自己的数量来定，能使用复孔的尽量做复孔。3、加样：①、空白孔：空白对照孔不加样品，生物素标记的抗32抗体，链霉亲和素HRP,只加显色剂4&K和终止液，其余各步操作相同;②、标准品孔：加入标准品50用，链霉素HRP5例l（标准品中已事先整合好生物素抗体，故不加）；③、待测样品孔：加入样本40Ml，然后各加入抗-TXB2抗体10诅、链酶亲和素HR尸50m，盖上封板膜，轻轻振荡混匀，37℃温育60分钟。④、配液：将浓缩洗涤液用蒸馏水稀释成1x应用液，备用。⑤、洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置30秒后弃去，如此重复5次，拍干。⑥、显色：每孔先加入显色剂A50出，再加入显色剂B50四1，轻轻震荡混匀，37℃避光显色10分钟。⑦、终止：每孔加终止液50出，终止反应（此时蓝色立转黄色）。⑧、测定：以空白空调零，450nm波长依序测量各孔的吸光度（OD值）。测定应在加终止液后10分钟以内进行。⑨、根据标准品的浓度及对应的OD值计算出标准曲线的回归方程，再根据样品的OD值在回归方程上计算出对应的样品浓度。也可以使用各种应用软件来计算。九、操作程序 总结 初级经济法重点总结下载党员个人总结TXt高中句型全总结.doc高中句型全总结.doc理论力学知识点总结pdf ：准备试剂，样品和标准品SZ加入准备好的样品和标准品，生物素标记二抗和酶标试剂，37℃反应60分钟洗板5次，加入显色液A、B，37℃显色10分钟加入终止液|10分钟之内读OD值计算检测范围：5pg/ml—1000pg/ml保存：2-8℃规格：96T/盒有效期：6个月（2-8℃）RatThromboxaneB2(TXB2)ElisaAssayKitInstructionSpeciesRatSamplesSerumBloodPlasmaStorage2-8℃ForresearchuseonlyNotforuseindiagnosticproceduresPleasecarefullyreadthisinstructionbeforeusing.ThisELISAkitisbasedontheprincipleofdouble-antibodysandwichtechniquetodetectRatThromboxaneB2(TXB2).Beusedonlyforresearchpurposes,notbeusedformedicaldiagnosis.1、INTENDEDUSEThiskitisusedtoassaytheThromboxaneB2(TXB2)inthesampleofRat’sserum,bloodplasmaandotherrelatedtissueLiquid.2、TESTPRINCIPLEThekitusesadouble-antibodysandwichenzyme-linkedimmuno-sorbentassay(ELISA)toassaythelevelofThromboxaneB2(TXB2)insamples.AddThromboxaneB2(TXB2)tomonoclonalantibodyEnzymewellwhichispre-coatedwithRatThromboxaneB2(TXB2)monoclonalantibody,incubation;then,addThromboxaneB2(TXB2)antibodieslabeledwithbiotin,andcombinedwithStreptavidin-HRPtoformimmunecomplex;thencarryoutincubationandwashingagaintoremovetheuncombinedenzyme.ThenaddChromogenSolutionA,B,thecoloroftheliquidchangesintotheblue,Andattheeffectofacid,thecolorfinallybecomesyellow.ThechromaofcolorandtheconcentrationoftheSubstanceThromboxaneB2(TXB2)ofsamplewerepositivelycorrelated.3、MATERIALSSUPPLIEDINTHETESTKITNumberItem48T96T1Standard(1280pg/ml)0.5ml0.5ml2Standarddiluent3ml3ml3MicroelisaStripplate12wellx4strips12wellx8strips4Str-HRP-ConjugateReagent3ml6ml5Washsolution20x20ml30x20ml6Biotin-TXB2Ab1ml1ml7ChromogenSolutionA3ml6ml8ChromogenSolutionB3ml6ml9StopSolution3ml6ml10Instruction1111Closureplatemembrane2(48T)2(96T)12Sealedbags114、MATERIALSREQUIREDBUTNOTSUPPLIED37℃incubatorStandardEnzymereaderPrecisionpipettesandDisposablepipettetips④Distilledwater⑤Disposabletubesforsampledilution⑥Absorbentpaper5、IMPORTANTNOTES1、Beeningtakenoutfromthe2-8℃environment,thekitshouldbebalanced30minutesintheambienttemperaturethenuse.IftheCoatedplatesofEnzymehaven’tbeenusedupafteropened,theremainingplatesshouldbestoredinSealedbag.2、Foreachstep,addSamplewithsampleinjectorwhichshouldbecalibratedfrequently,inordertoavoidunnecessaryexperimentaltolerance.3、Theoperationshallbecarriedoutaccordancetotheinstructionsstrictly.AndtestresultsmustbebasedonthereadingsoftheEnzymereader.4、Inordertoavoidcross-contamination,itisforbiddentore-usethesuctionheadandsealplatemembraneinyourhands.5、Theidleagentsshallbeputuporcovered.Donotusereagentwithdifferentbatches.Andusethembeforeexpireddate6、ThesubstrateBislight-sensitive.Prolongedexposuretolightisforbidden.6、WASHINGMETHODManuallywashingmethod:shakeawaytheremainliquidintheenzymeplates;placesomebibulouspapersonthetest-bed,andflaptheplatesontheupsidedownstrongly.Injectatleast0.35mlafter-dilutionwashingsolutionintothewell,andmarinate1~2minutes.Repeatthisprocessaccordingtoyourrequirements.Automaticwashingmethod:ifthereisautomaticwashingmachine,itshouldonlybeusedinthetestwhenyouarequitefamiliarwithitsfunctionandperformance.7、SPECIMENREQUIREMENTS1、Don’tdetectthesampleswithNaN3whichcouldinhibitHRPactivityofthehorseradishperoxidase.2、ExtractassoonaspossibleafterSpecimencollection.Theextractionshouldconformwiththerelevantliterature,andthencarryouttheexperimentassoonaspossible.Ifitisimpossibletotestimmediately,preservethespecimenin-20℃enviroment.Andrepeatedfreezingandthawingisforbidden.8、ASSAYPROCEDURE1、Standarddilution:(ThistestkitwillsupplyoneoriginalStandardreagent,pleasediluteitbyyourselfaccordingtotheinstruction.)640pg/mlStandardNo.5120见OriginalStandard+120出Standarddiluents320pg/mlStandardNo.4120出StandardNo.5+120^1Standarddiluents160pg/mlStandardNo.3120^1StandardNo.4+120^1Standarddiluents80pg/mlStandardNo.2120^1StandardNo.3+120^lStandarddiluents40pg/mlStandardNo.1120^lStandardNo.2+120^lStandarddiluents2、Thequantityoftheplatesdependsonthequantitiesofto-be-testedsamplesandthestandards.Itissuggestedtoduplicateeachstandardandblankwell.Everysampleshallbemadeaccordingtoyourrequiredquantity,andtrytousetheduplicatedwellaspossible.3、Injectsamples:Blankwell:don’taddsamplesandTXB2-antibodylabeledwithbiotin,Streptavidin-HRP,onlyChromogensolutionAandB,andstopsolutionareallowed;otheroperationsarethesame.Standardwells:addstandard50^1,Streptavidin-HRP50出(sincethestandardalreadyhascombinedbiotinantibody,itisnotnecessarytoaddtheantibody);Tobetestwells:addsample40^1,andthenaddbothTXB2-antibody10^1andStreptavidin-HRP50^1.Thensealthesealingmemberance,andgentlyshaking,incubated60minutesat37℃.Confection:dilutethewashsolutionwithdistilledwaterto1xworkingsolutionforuse.Washing:removethememberancecarefully,anddraintheliquid,shakeawaytheremainingwater.AddchromogensolutionA50^l,thenchromogensolutionB50^ltoeachwell.Gentlymixed,incubatefor10minat37℃awayfromlight.Stop:AddStopSolution50^lintoeachwelltostopthereaction(thebluechangesintoyellowimmediately).Finalmeasurement:Takeblankwellaszero,measuretheopticaldensit(OD)under450nmwavelengthwhichshouldbecarriedoutwithin10minafteraddingthestopsolution.Accordingtostandards’concentrationandthecorrespondingODvalues,calculateoutthestandardcurvelinearregressionequation,andthenapplytheODvaluesofthesampleontheregressionequationtocalculatethecorrespondingsample’sconcentration.Itisacceptabletousekindsofsoftwaretomakecalculations.9、SUMMARYPROCEDURESPreparingreagents,samplesandstandardsAddpreparedsamplesandstandards,antibodieslabeledwithenzyme,reacting60minutesat37℃Platewashedfivetimes,addingChromogenrsolutionA,B,reacting10minutesat37℃。AddstopsolutionRmeasuretheODvaluewithin10minxzPackagesize：96TperboxValidity：Sixmonths(2-8℃)CalculationAssayrange：5pg/ml-l000pg/mlStorage：2-8℃