九种蝗虫精子发生过程中ckit蛋白特异性表达比较分析英文

九种蝗虫精子发生过程中ckit蛋白特异性 表 关于同志近三年现实表现材料材料类招标技术评分表图表与交易pdf视力表打印pdf用图表说话 pdf 达比较 分析 定性数据统计分析pdf销售业绩分析模板建筑结构震害分析销售进度分析表京东商城竞争战略分析 英文 ) ( 2006, 2007, 2009 . T h e s tage2sp ec ific exp ress io n o f n o r th eas te rn a rea o f C h in a Z h en g et a .l , 1990; R en, c2k it p ro te in p ro v ed th a t th e c2k it p ro te in fu n c tio n n o t ) 2001 an d h ad s im ila r liv in g en v iro nm en t w h e re th ey o n ly in m am m a l ge rm ce ll p ro life ra tio n b u t a lso in sh o u ld sh a re th e sp ace, th e fo o d an d th e o th e r ) in sec t sp e rm a to gen es is. ( 2004 . T o av o id th e eco lo g ica l so u rces Z h ao et a l. , m igh t d iffe ren tia te in T h e n in e g rassh op p e r sp ec ies in c lu d ed C a llip tamus com p e tin g in life cy c le, th ey ( ( ) ) rep ro d u c tiv e abbrevia tus Ik o n n. , S hirakiacris shirakii I.s tru c tu re, d ev e lopm en ta l tim e, B o . l , p h y s io lo g ica l Omocestus viridu lus L in n aeu s, E uchorthippus unicolor lev e l, ev en th e p ro te in exp ress io n an d ( ) 2k it o n p rev io u s resea rch es o n cIk o n n. ,E uchorthippus vitta tus Z h en g, Epacrom ius regu la tio n. B as in g ( ) ( ) coeru lipes Iv an. , A iolopus tha lassinus tamu lus Fab r. , p ro te in in g rassh op p e rs, w e fu r th e r com p a red an d O eda leus inferna lis Sau ssu re, T rilophid ia annu la ta an a ly sed th e d iffe ren ces o f c2k it p ro te in exp ress io n in T h u n b e rg w h ich b e lo n ged to th ree fam ilies, n am e ly sp e rm a to gen es is am o n g d iffe ren t sp ec ies. W e a im ed to rev ea l th e m ean in gfu l ac tio n o f c2k it p ro te in in C a tan top id ae, O ed ip o d id ae an d A rcyp te r id ae ( ) ( rep ro d u c tiv e iso la tio n, esp ec ia lly b e tw een re la tiv e O r th op te ra, A c r id o id ea th e tax o n om ic ca tego ry sp ec ies. ) sh ow ed as fo llow in g. T h ey w e re com m o n sp ec ies in ) (F ig. 1. R e la tion sh ip tree o f n in e sp ec ies g rassh opp e rs from J ilin P ro v in ce, C h n ia B ased o n Z h en g, 1990. ) 2 M a te r ia l a n d M e th o d s an ti2rab b it p o ly c lo n a l an tib o d y d ilu ted 1 ?200 in TB S co n ta in in g 0105 % ace ty la ted B SA. T h en, afte r aga in 211 In sec ts r in s in g tw ice w ith PB S, th e s lid es w e re s ta in ed w ith N in e sp ec ies o f g rassh op p e rs ad u lts w e re cau gh t ( ) 0105 % w / v 3 ′3 2d iam in o b en zid in e te trach lo r id e in ran d om ly in sum m e r from S ip in g A rea o f J ilin ( ) 0105 m m o l /L T r is2H C l b u ffe r p H 716 co n ta in in g P ro v in ce, C h in a. Tw en ty tes tes o f n in e sp ec ies ( ) 1 % v / v H O 3 m in an d r in s in g tw ice w ith PB S. C 2 2 g rassh op p e rs w e re d issec ted in n o rm a l sa lin e. T h e o n tro l sec tio n s, in w h ich th e p r im a ry an tib o d y w as tissu es w e re fix ed in B o u in so lu tio n, th en em b ed d ed in e ith e r om itted, w e re s im ila rly p ro cessed. A ll μp a raffin. A ll sec tio n s w e re cu t a t a th ick n ess o f 6m , in cu b a tio n s tep s w e re ca r r ied o u t in a m o is t ch am b e r. m o u n ted, an d d ep a raffini zed. F in a lly, th e sec tio n s w e re d eh y d ra ted an d m o u n ted. 212 Im m u n o h is to ch em is try A ll b io lo g ica l reagent u sed ab o v e p ro cess w e re b o u gh t b lo ck th e en d o gen o u s p e ro x id ase ac tiv ity, th e T o from W u h an B o s te r L td. co. , C h in a. ( ) sec tio n s w e re in cu b a ted w ith 3 % v / v h y d ro gen 213 M ea su rem en t a n d p h o to g rap h ( ) p e ro x id e H O in m e th an o l fo r 10 m in. T o u nm ask 2 2 W e se t fo u r rep resen ta tiv e p h ases in th e ep itop es, w e h ea ted th e sec tio n s a t 95 ? in a 0101 sp e rm a to gen es is to d e tec t th e exp ress io n lev e l o f c2k it ( ) m m o l /L so d ium c itra te2b u ffe r sa lin e p H 610 fo r 10 p ro te in. 10 sec tio n s o f tes tis w e re se lec ted fo r each m in s in m ic row av e o v en, rep ea ted afte r 10 m in an d sec tio n 10 v isu a l sp ec ies, an d fo r each g rassh op p e r co o led sec tio n s a t ro om tem p e ra tu re. A fte r r in s in g fie ld s w e re se lec ted. P e rcen tage an d av e rage a rea o f ( tw ice w ith 011 m m o l /L p h o sp h a te 2b u ffe r sa lin e PB S, c2k it p ro te in to rep resen t th e p o s itiv e g ran u le o f ) p H 712 - 716 , th e sec tio n s w e re b lo ck ed w ith 10 % exp ress io n in ten s ity o f c2k it p ro te in in each s tage o f n o rm a l go a t se rum fo r 30 m in. T h e ex cess b lo ck in g g rassh op p e r sp e rm a to gen es is w e re m easu red an d b u ffe r w as th en rem o v ed from th e s lid es b efo re p h o to g rap h ed b y M o tic Im ages A d v an ced 312 u n d e r ( in cu b a tin g th em w ith th e p r im a ry an tib o d y 1 ?100 400 tim es v isu a l fie ld s. )m o n o c lo n a l an ti2rab b it c2k it recep to r an tib o d y o v e rn igh t a t 4 ?. A fte r th is in cu b a tio n, th e s lid es w e re214 S ta tis tica l a n a ly s is r in sed tw ice w ith PB S an d in cu b a ted fo r 20 m in a t S tan d a rd s ta tis tica l m e th o d s w e re u sed to es tim a te ( 37 ? w ith th e seco n d an tib o d y a b io tin y la ted go a tth e m ean an d v a r ian ce o f sam p le d a ta. A ll d a ta w e re th e an a ly zed u s in g AN O V A ; S 2N 2K an d L SD tes ts w e re n in e sp ec ies o f g rassh op p e rs. In C a tan top id ae, ( I. u sed to m ak e m u ltip le com p a r iso n s. T h e s ta tis tic d a ta shirakii p o s itiv e p e rcen tage o f c2k it p ro te in in S. ( ) sh ow ed in p ap e r w e re m ean ?S E n = 20 an d P v a lu e th a t in C. ) B o l. w e re s ign ifican tly h igh e r th an 3 ( ) ( ) < 0105 abbrevia tus Ik o n n. . w e re co n s id e red to b e s ign ificant. A ll In A rcyp te r id ae, th e p o s itiv e 1310 p e rcen tage o f c2k it p ro te in ca r r ied o u t b y u s in g S PSS in O. viridu lus L in n aeu s an d an a ly ses w e re ( ) E u. so ftw a re. unicolor Ik o n n. w e re s ign ifican tly h igh e r th an th a t in E u. vitta tus Z h en g. In O ed ip o d id ae, th e 3 R esu lts p o s itiv e p e rcen tage o f c2k it p ro te in w as low es t in E. exp ress io n o f th e c2k it p ro te in in 311 Sp ec ia l ( ) coeru lipes Iv an. an d h igh es t in A. tha lassinus tamu lus ( )sp e rm a to go n ia F igs 2 - 13 ( ) Fab r. w h ile th e re w e re n o t s ign ifican t d iffe ren ces th e p o s itiv e p e rcen tage o f c2k it F rom o te in p rinferna lis Sau ssu re an d T. annu la ta b e tw een O. ( ) F ig. 12 , th e re w e re s ign ificant d iffe ren ces am o n g T h u n b e rg. ) ( F igs 2 - 11. Im m u n o h is to ch em ica l p h o to of c2k it p ro te in exp ress io n in sp e rm a togo n ia am o n g n in e sp ec ies g rassh opp e rs 400 ×, ( ) in w h ich th e n um be rs from 2 to 10 rep resen t d iffe ren t sp ec ies g rassh opp e r as fo llow. 2. C. abbrevia tus Ik o n n. . 3. S. shirakii ( ) ( ) ( ) I. B o . l . 4. O. viridu lus L in n aeu s. 5. E. coeru lipes Ivan. . 6. Eu. vitta tus Z h en g. 7. Eu. unicolor Ik on n. . 8. A. tha lassinus ( ) ( ) tamu lus Fab r. . 9. O. inferna lis Sau ssu re. 10. T. annu la ta T h u n b e rg. 11. C o n tro l of C. abbrevia tus Iko n n. . 12. P e rcen tage 2 ( ) 2k it p ro te in % . μ) of p o s itive g ran u les o f c( 13. A rea of p o s itive g ran u les o f c2k it p ro te in m . D a ta w e re exp ressed as M ean ? ) ( tes t, n = 20. T h e re w e re s ign ifican t d iffe ren ces am on g th e co n ten ts w ith th e d iffe ren t SE, O n e2w ay AN O V A , S 2N 2K an d L SD ( ) sm a ll le tte rs P < 0105an d n o s ign ifican t d iffe ren ces am o n g th e co n ten ts w ith th e sam e sm a ll le tte rs. F rom th e a rea o f p o s itiv e g ran u les o f c2k it p ro te in n o t s ign ifican t d iffe ren ces b e tw een C. abbrevia tus ( ) ( ) ( ) Ik o n n. an d S. shirakii I. B o . l . In A rcyp te r id ae, F ig. 13 , th e re w e re s ligh t d iffe ren ces am o n g n in e th e p o s itiv e g ran u les in E u. vitta tus Z h en g w e re sm a lle r sp ec ies o f g rassh op p e rs. In C a tan top id ae, th e re w e re ) ( 313 Sp ec ia l exp ress io n o f th e c2k it p ro te in in Ik o n n. . In A rcyp te r id ae, th e p o s itiv e p e rcen tage o f ( )seco n d a ry sp e rm a to cy te F igs 26 - 37 c2k it p ro te in h ad n πt s ign ifican t d iffe ren ces am o n g O. F rom th e p o s itiv e p e rcen tage o f c2k it p ro te in ( ) viridu lus L in n aeu, E u. unicolor Ik o n n. an d E u. ( ) vitta tus Z h en g. In O ed ip o d id ae, th e p o s itiv e p e rcen tage F ig. 36 , th e re w e re d iffe ren t exp ress io n am o n g n in e o f c2k it p ro te in ( ) sp ec ies o f g rassh op p e rs. In C a tan top id ae, th e p o s itiv e in A. tha lassinus tamu lus Fab r. w e re ) ( ( ) p e rcen tage o f c2k it p ro te in in S. shirakii I. B o . l w e re h igh es t th an th a t in E. coeru lipes Iv an. ,O. inferna lis abbrevia tus s ign ifican tly low e r th an th a t in C. S au ssu re an d T. annu la ta T h u n b e rg. F igs 26 - 35. Im m u n o h is toch em ica l p h o to o f c2k it p ro te in exp ress ion in secon d a ry sp e rm a to cy te am o n g n in e sp ec ies g rassh op p e rs ( ) ( ) 400 ×, in w h ich th e n um b e rs from 26 to 34 rep resen t d iffe ren t sp ec ies g rassh op p e r as fo llow. 26. C. abbrevia tus Ik o n n. . ( ) ( ) 27. S. shirakii I. B o . l . 28. O. viridulus L in n aeu s. 29. Eu. unicolor Ik on n. . 30. Eu. vitta tus Z h en g. 31. E. coeru lipes ( ) ( ) Iv an. . 32. A. tha lassinus tamu lus Fab r. . 33. O. inferna lis Sau ssu re. 34. T. annula ta T h u n be rg. 35. C o n tro l of C. abbrevia tus 2 ( ) μ) ( ) (Ik o n n. . 36. P e rcen tage o f p os itiv e g ran u les of c2k it p ro te in % . 37. A rea o f p o s itive g ran u les o f c2k it p ro te in m . D a ta ) ( w e re exp ressed as M ean ?SE, O n e2w ay AN O V A , S 2N 2K an d L SD tes t, n = 20. T h e re w e re s ign if ican t d iffe ren ces am on g th e ( ) con ten ts w ith th e d iffe ren t sm a ll le tte rs P < 0105 an d n o s ign if ican t d iffe ren ces am on g th e co n ten ts w ith th e sam e sm a ll le tte rs. ( ) F rom th e a rea o f p o s itiv e g ran u les o f c2k it p ro te in p e rcen tage o f c2k it p ro te in in S. shirakii I. B o l. w e re ( ) F ig. 37 , th e re w e re s ligh t d iffe ren ces am o n g n in e s ign ificant ly low e r th an th a t in C. abbrevia tus sp ec ies o f g rassh op p e rs. In C a tan top id ae, th e re w e re ( ) Ik o n n. . In A rcyp te r id ae, th e p o s itiv e p e rcen tage o f c2k it p ro te in w e re s tro n ges t in O. viridu lus L in n aeu s, s n o t s ign ificant d iffe ren ces b e tw een C. abbrevia tus ( ) ( ) Ik o n n. an d S. shirakii I. B o l. . In A rcyp te r id ae, ) ( tro n ge r in E u. unicolor Ik o n n. an d sm a lles t in E u. th e p o s itiv e g ran u les w e re b igges t in O. viridu lus vitta tus Z h en g. In O ed ip o d id ae, th e p o s itiv e p e rcen tage an d sm a lles t ( ) L in n aeu s, b igge r in E u. unicolor Ik o n n. ( ) o f c2k it p ro te in in A. tha lassinus tamu lus Fab r. w e re in E u. vitta tus Z h en g. In O ed ip o d id ae, th e p o s itiv e ( ) h igh es t th an th a t in E. coeru lipes Iv an. , O. inferna lis ( ) g ran u les in E. coeru lipes Iv an. an d annu la ta T. S au ssu re an d T. annu la ta T h u n b e rg. tha lassinus T h u n b e rg w e re sm a lle r th an th a t in A. F rom th e a rea o f p o s itiv e g ran u les o f c2k it p ro te in ( ) tamu lus Fab r. an d O. inferna lis S au ssu re. ( ) F ig. 49 , th e re w e re s ligh t d iffe ren ces am o n g n in e sp ec ies o f g rassh op p e rs. In C a tan top id ae, th e p o s itiv e 314 Sp ec ia l exp ress io n o f th e c2k it p ro te in in ( ) g ran u les in C. abbrevia tus Ik o n n. w e re sm a lle r th an )( m a tu re sp e rm F igs 38 - 49 ( ) A rcyp te r id ae, th e I. B o . l . Inth a t in S. shirakii F rom th e p o s itiv e p e rcen tage o f c2k it p ro te in p o s itiv e g ran u les w e re b igges t in O. viridu lus L in n aeu s, ( ) F ig. 48 , th e re w e re d iffe ren t exp ress io n am o n g n in e sp ec ies o f g rassh op p e rs. In C a tan top id ae, th e p o s itiv e ) ( F igs 38 - 47. Im m u n o h is toch em ica l p h o to of c2k it p ro te in exp ress io n in m a tu re sp e rm am on g n in e sp ec ies g rassh opp e rs 400 ×, ( ) in w h ich th e n um be rs f rom 38 to 46 rep resen t d iffe ren t sp ec ies g rassh op p e r as fo llow. 38. C. abbrevia tus Ik on n. . 39. S. shirakii ( ) ( ) ( ) I. B o . l . 40. O. viridu lus L in n aeu s. 41. Eu. unicolor Ik on n. . 42. E. coerulipes Ivan. . 43. Eu. vitta tus Z h en g. 44. A. ( ) ( ) tha lassinus tamu lus Fab r. . 45. O. inferna lis Sau ssu re. 46. 47. C on tro l of C. abbrevia tus Ik o n n. . T. annula ta T h u n b e rg. 2 μ) ( ) (48. P e rcen tage o f p o s itive g ran u les o f c2k it p ro te in % . 49. A rea of p os itiv e g ran u les of c2k it p ro te in m . D a ta w e re) ( exp ressed as M ean ?SE, O n e2w ay AN O V A , S 2N 2K an d L SD tes t, n = 20 . T h e re w e re s ign ifican t d iffe ren ces am o n g th e ( ) con ten ts w ith th e d iffe ren t sm a ll le tte rs P < 0105 an d n o s ign if ican t d iffe ren ces am on g th e co n ten ts w ith th e sam e sm a ll le tte rs. ( )( ) an d sm a lles t in E u. 2k it p ro te in in S. shirakii I. B o l. w e re p e rcen tage o f cb igge r in E u. unicolor Ik o n n. th e p o s itiv e g ran u les vitta tus Z h en g. In O ed ip o d id ae, s ign ifican tly low e r th an th a t in C. abbrevia tus ( ) Ik o n n. . In A rcyp te r id ae, th e p o s itiv e p e rcen tage o f ( ) in A. tha lassinus tamu lus Fab r. w e re b ige r th an th a t in ( ) coeru lipes Iv an. , O. inferna lis Sau ssu re an d E. c2k it p ro te in in O. viridu lus L in n aeu an d E u. unicolor T. ( ) annu la ta T h u n b e rg. Ik o n n. w e re h igh e r th an th a t in E u. vitta tus Z h en g. th e p o s itiv e p e rcen tage o f c2k it In O ed ip o d id ae, 315 Sp ec ia l exp ress io n o f th e c2k it p ro te in in th e ( ) tha lassinus p ro te in in E. coeru lipes Iv an. an d A.( )en d o f sp e rm a ry F igs 50 - 61 ( ) w e re h igh e r th an th a t inferna lis tamu lus Fab r. in O. 2k it p ro te in F rom th e p o s itiv e p e rcen tage o f cannu la ta T h u n b e rg. S au ssu re an d T. ( ) F ig. 60 , th e re w e re d iffe ren t exp ress io n am o n g n in e sp ec ies o f g rassh op p e rs. In C a tan top id ae, th e p o s itiv e in th e en d o f sp e rm a ry am on g n in e sp ec ies g rassh op p e rs F igs 50 - 59. Im m u n oh is to ch em ica l p h o to of c2k it p ro te in exp ress io n ( ) ( ) 400 ×, in w h ich th e n um b e rs from 50 to 58 rep resen t d iffe ren t sp ec ies g rassh op p e r as fo llow. 50. C. abbrevia tus Ik o n n. . ( ) ( ) 51. S. shirakii I. B o . l . 52. O. viridulus L in n aeu s. 53. Eu. unicolor Ik on n. . 54. Eu. vitta tus Z h en g. 55. E. coeru lipes ( ) ( ) Iv an. . 56. A. tha lassinus tamu lus Fab r. . 57. O. inferna lis Sau ssu re. 58. T. annula ta T h u n be rg. 59. C o n tro l of C. abbrevia tus 2 ( ) μ) ( ) (Ik o n n. . 60. P e rcen tage o f p os itiv e g ran u les of c2k it p ro te in % . 61. A rea o f p o s itive g ran u les o f c2k it p ro te in m . D a ta ) ( w e re exp ressed as M ean ?SE, O n e2w ay AN O V A , S 2N 2K an d L SD tes t, n = 20. T h e re w e re s ign if ican t d iffe ren ces am on g th e ( ) con ten ts w ith th e d iffe ren t sm a ll le tte rs P < 0105 an d n o s ign if ican t d iffe ren ces am on g th e co n ten ts w ith th e sam e sm a ll le tte rs. F rom th e a rea o f p o s itiv e g ran u les o f c2k it p ro te in th e p o s itiv e g ran u les in O. viridu lus L in n aeu s w e re ( ) F ig. 61 , th e re w e re s ligh t d iffe ren ces am o n g n in e ( ) b igge r th an th a t in E u. unicolor Ik o n n. an d E u. sp ec ies o f g rassh op p e rs. In C a tan top id ae, th e re w e re vitta tus Z h en g. In O ed ip o d id ae, th e p o s itiv e g ran u les ( ) n o t s ign ifican t d iffe ren ces b e tw een C. abbrevia tus in E. coeru lipes Iv an. an d A.tha lassinus tamu lus ( ) ( ) ( ) Ik o n n. an d S. shirakii I. B o . l . In A rcyp te r id ae, Fab r. an d w e re sm a lle r th an th a t in O. inferna lis G u e r if, F. , C ad o re t, V. , R ah a l2P e ro la, V. , L an sac, J. , B e rn ex, F. , S au ssu re an d T. annu la ta T h u n b e rg. Jacqu es P an th ie r, J. , H och e reau 2d e R ev ie rs, M . T. an d R oye re, 4 D iscu ss io n D. 2002. A p op tos is, on se t an d m a in ten an ce of sp e rm a togen es is, ev id en ce fo r th e in vo lvem en t of K it in K it2h ap lod efic ien t m ice. th e re a re m illio n s o f sp ec ies o f In in sec ts, in sec ts ( ) B iology of R ep roduction, 67 1 : 70 - 79. K isse l, H. , T im okh in a, I. , H a rd y, M . P. , R o th sch ild, G. , T a jim a, an d m an y sp ec ies o f in sec ts a re k eep in g c lo se Y. , Soa res, V . , A n ge les, M . , W h itlow , S. R. , M an ova, K. an d re la tio n sh ip. A s an in d ep en d en t sp ec ies, th ey m u s t B esm e r, P. 2000. P o in t m u ta tion in k it recep to r ty ros in e k in ase k eep sex u a l iso la tio n w ith o th e r sp ec ies, in c lu d in g revea ls essen tia l ro les fo r k it s ign a lin g in sp e rm a togen es is an d g lan d, m a tin g seaso n, th e s tru c tu re o f sex u a l oogen es is w ith ou t affectin g o th e r k it resp on ses. EM BO J ourna l, 19 ( ) A fte r 6 : 1 312 - 1 326. p h y s io lo g ica l p e r io d an d ev en p ro te in lev e l. L i, J, X ia, F an d L i, W 2X 2003. C oac tiva tion of STA T an d R as is s im ila r im m u n o h is to ch em ica l s ta in in g, th e re a re requ ired fo r ge rm ce ll p ro life ra tion an d in vas ive m ig ra tion in exp ress io n ten d en cy o f c2k it p ro te in in fo u r s tages o f ( ) D rosophila. D evelopmenta l Cell, 5 5 : 787 - 798. sp e rm a to gen es is o f n in e sp ec ies g rassh op p e rs. A fte r w e O h ta, H. , T oh d a, A. an d N ish im u n e, Y. 2003. P ro life ra tion an d d iffe ren tia tion of sp e rm a togon ia l s tem ce lls in th e w /w v m u tan t fu r th e r com p a re an d an a ly se th e d iffe ren ces o f c2k it ( ) m ou se tes tis. B iology of R ep roduction, 69 6 : 1 815 - 1 821. p ro te in exp ress io n, th e resu lts sh ow th a t th e re a re P rasan th, S. G. an d A li, S. 2003. Exp ress ion of p ro to 2on cogen e c2k it m o re com p lica ted ch an ges in tim e an d sp ace d u r in g ( ) recep to r in ra ts R a ttu s n o rveg icu s an d id en tifica tion of a m u tan t sp e rm a to gen es is am o n g n in e sp ec ies g rassh op p e rs, m RN A tran sc r ip t im p lica ted in sp e rm a togen ic fa ilu re. DNA Cell ( ) B iology, 22 7 : 447 - 456. esp ec ia lly in re la tiv e sp ec ies. In C a tan top id ae, th e R en, B 2Z 2001. G rassh opp e rs an d L ocu s ts from N o r th eas t. J ilin p e rcen tage o f c2k it p ro te in g ran u les b e tw een S. shirakii Sc ien tific an d T ecch on log ica l P ress. 14 - 123. ( ( )) I. B o l. an d C. abbrevia tus Ik o n n. a lw ay s sh ow R oss i, P. , D o lc i, S. , Se tte, C. an d G e rem ia, R. 2003. M o lecu la r s ign ifican t d iffe ren ces in ev e ry s tage o f m ech an ism s u tilized by a lte rn a tive c2k it gen e p rod u cts in th e con tro l of sp e rm a togon ia l p ro life ra tion an d sp e rm 2m ed ia ted egg ac tiva tion. sp e rm a to gen es is. In A rcyp te r id ae, th e E u. unicolor ( ) A ndrologia, 35 1 : 71 - 78. ( ) Ik o n n. an d th e E u. vitta tus Z h en g a re m o re c lo seR oss i, P. , Se tte, C. , D o lci, S. an d G e rem ia, R. 2000. R o le of c2k it in re la tiv es th an th e O. viridu lus L in n aeu b u t th ey ap p ea r ( ) m am m a lian sp e rm a togen es is. J ourna l of Endocrinol Invest, 23 9 : g rea t d iffe ren ces in th e b egg in n in g o f sp e rm a to gen es is. In 609 - 615. ( ) O ed ip o d id ae, A. tha lassinus tamu lus Fab r. sh ow San d low , J. I. , Fen g, H 2L an d San d ra, A. 1997. L oca liza tion an d exp ress ion of th e c2k it recep to r p ro te in in h um an an d rod en t tes tis d iffe ren ces o f c2k it p ro te in exp ress io n th an th a t o f th ree ( ) an d sp e rm . U rology, 49 3 : 494 - 500. sp ec ies in p e r io d s o f sp e rm a to go n ia, seco n d a ry Se tte, C. , D o lci, S. , G e rem ia, R. an d R oss i, P. 2000. T h e ro le of ( ) sp e rm a to cy te an d sp e rm w h ile E. coeru lipes Iv an. s tem ce ll fac to r an d of a lte rn a tive c2k it gen e p rod u c ts in th e exp ress low e r c2k it p ro te in in sp e rm a to go n ia an d O. es tab lishm en t, m a in ten an ce an d fu n c tion of ge rm ce lls. Interna tiona l ( ) J ouna l of D evelopmenta l B iology, 44 6 : 599 - 608. inferna lis Sau ssu re an d T. annu la ta T h u n b e rg a lso sh ow U n n i, S. K. , M od i, D . N. , P a th ak, S. G. , D h aba lia, J. V. an d th e d iffe ren ces o f c2k it p ro te in g ran u les a rea in B h a r tiya, D. 2009. S tage2sp ec ific loca liza tion an d exp ress ion of c2 seco n d a ry sp e rm a to cy te p e r io d. F rom th e d y n am ic k it in th e ad u lt h um an tes tis. J . H istochem Cytochem, 57: 861 - 869. ch an ges in d iffe ren t s tages an d exp ress io n lev e ls, th e c2 V igod n e r, M . , L ew in, L. M . , Sh och a t, L. an d G o lan, R. 2001. Sp e rm a togen es is in th e go ld en h am s te r, th e ro le of c2k it. M olecula r k it p ro te in m igh t p a r tic ip a te in co n tro llin g th e tim e an d ( ) R ep roduction and D evelopment, 60 4 : 562 - 568. sp ace s tep in sp e rm a to gen es is o f g rassh op p e rs. F in a lly Z h ao, Z, L i, X 2K, Z h ou, X 2F, H ao, X 2L an d X u, H 2W 2009. T h e th e d iffe ren ces o f c2k it p ro te in exp ress io n resu lt in th e sp ec ia l exp ress ion an d com p a r ison of th e c2k it p ro te in in p e r io d in sp ece is esp ec ia lly in d iffe ren t rep ro d u c tiv e gam e togen es is be tw een Euchorthippus vitta tus Z h en g an d Euchorthippus ( ) unicolor Ikon n. . IEE E: 1 388 - 1 392. re la tiv e sp ec ies. Z h ao, Z, L v, S 2M an d X i, G 2S 2006. T em p o ra l exp ress ion of c2k it in c2 A ll in a ll, as an im p o r tan t regu la tin g p ro te in, sp e rm a togen es is of tw o g rassh opp e rs sp ec ies. Insect S cience, 13: 445 k it p ro te in m igh t b e p lay in g a k ey ro le in th e p ro cess o f - 450. sp e rm a to gen es is in g rassh op p e rs. W h ile in d y n am ic Z h ao, Z, R en, B 2Z an d X i, G 2S 2005. Sp e rm a togen es is in Omocestus ( ) viridulus. J ourna l of J ilin A gricultura l U niversity, 27 6 : 603 - 605. exp ress io n o f c2k it p ro te in in sp e rm a to gen es is am o n g Z h ao, Z an d X i, G 2S 2007. T h e sp ec ia l exp ress ion an d com p a r ison of g rassh op p e rs, w e n eed m o re ev id en ces to p ro v e th a t th e c2k it P ro te in in sp e rm a togen es is of th ree sp ec ies of locu s ts of th e c2k it p ro te in m a in ta in th e sp ec ies d iffe ren ces, ( ) A rcyp te r id ae. A gricultura l S cience in China, 6 7 : 825 - 831. esp ec ia lly in re la tiv e sp ec ies. Z h ao, Z, Yu, Y2P an d R en, B 2Z 2004. S tu d y on T h e com p os ion an d season a l d yn am ic of locu s t com m u n ities in S ip in g su bu rb of J ilin R E FER EN C ES ( ) p rov in ce, C h in a. J ourna l of J ilin A gricultura l U niversity, 26 3 : 267 - 271. 2000. S tem ce ll fac to r / c2k it Fen g, L , R av in d ran a th, N. an d D ym , M . Z h en g, Z 2M , X u, W 2X an d L ian, Z 2M 1990. Sh aan x i G rassh opp e r. up 2regu la tes cyclin D 3 an d p rom o tes ce ll cyc le p rog ress ion v ia th e Sh aan x i N o rm a l U n ive rs ity P ress, X iπan. 47 - 144. p h osp h o in os itid e 3 2k in ase / p 70 S6 k in ase p a thw ay in sp e rm a togon ia. ( ) J ourna l B iologica l Chem istry, 275 33 : 25 572 - 25 576. 3 九种蝗虫精子发生过程中 c 2k i t 蛋白特异性表达比较分析 1 2123 3 赵张婷婷 郑哲民 卓 奚耕思 1. 吉林师范大学生命科学学院 四平 1360002. 陕西师范大学生命科学学院 西安 710062 摘 要 c2k it蛋白在昆虫和哺乳动物生殖细胞发育中发挥着 c2k i蛋t 白表达差异 。结果显示 , 蝗虫精子发生过程中 c2k it蛋 9种蝗虫种间 c2k it蛋白表 并且在 重要作用 。本研究通过免疫组织化学法和生物统计方法 , 比 白均出现阶段特异性表达 , 较分析了隶属于直翅目蝗总科的 9 种蝗虫精子发生过程中 达出现显著差异 。关键词 c2k it蛋白 , 精子发生 ,蝗虫 ,种间差异. Q 965 中图分类号 3 本文为祝贺郑哲民教授执教 60 周年暨 80 寿辰而作. 3 3 通讯作者 , E 2m a il: x igen gs i@ sn n u. ed u. cn file:///D|/我的资料/Desktop/新建文本文 档.txt Appliance Error (configuration_error) Your request could not be processed because of a configuration error: "Could not connect to LDAP server." For assistance, contact your network support team. file:///D|/我的资料/Desktop/新建文本文档.txt2012-07-12 20:42:52