培养基灵敏度检查规程
目的:
建立培养基灵敏度检查的操作规程，保证无菌检查结果的准确性可靠性。
2. 范围:
本
标准
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适用于培养基灵敏度检查。
3.
职责
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:
QC无菌检验员对本标准的实施负责。
4. 程序:
4.1. 实验设备及用具:
高压蒸汽灭菌器、恒温培养箱、注射器、针头、注射器盒、试管、试管架、搪瓷托盘、时钟、75%酒精棉球、无菌服。
4.2. 培养基:
需气菌、厌气菌培养基(流体硫乙醇酸盐培养基)、真菌培养基。
4.3. 测试用菌种
4.3.1. 藤黄微球菌[CMCC(B)28 001]
4.3.2. 生孢梭菌[CMCC(B)64 941]
4.3.3. 白色念珠菌[CMCC(F)98 001]
4.4. 操作:
4.4.1. 取藤黄微球菌、生孢梭菌的需气菌、厌气菌培养基新鲜培养物，白色念珠菌 的真菌培养基的新鲜培养物1ml，用0.9%无菌氯化钠溶液作10倍系列稀释，制成1ml中含10-100个菌并计数。
4.4.2. 将藤黄微球菌、生孢梭菌的上述稀释液各1ml分别接种至每管装量为9ml的需practices. The development of various undertakings, requires each party to pay and hard work. No free ride thing under the Sun, if you cannot play a vanguard and exemplary role of party members is unworthy party members. Both staff members and no staff members, both vigorous and resolute working style, good at policy advocacy, and to perform good decisions of party committees and Governments, determined to complete work assigned by the superior party. Third, focus on improving service, the ability
气菌、厌气菌培养基3管，白色念珠菌的上述稀释菌液1ml，接种至每管装量为9ml的真菌培养基3管。以未接种的培养基作对照，按规定的温度培养5天并逐日记录结果。
4.5. 结果判定:以每株菌接种后的培养基不得少于2管呈现生长，即该培养基的灵敏度检查符合要求。
5. 附:
培养基灵敏度检查记录ZL0081 00。
压力蒸汽灭菌化学指示卡使用规程
目的:
建立压力蒸汽灭菌化学指示卡使用规程，保证正确使用，对灭菌效果进行有效监测。
2. 依据:
《压力蒸汽灭菌化学指示卡使用
说明书
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》。
3. 范围:
本标准适用于压力蒸汽灭菌化学指示卡对压力蒸汽灭菌(121?，30分钟)的灭菌效果进行的监测。
4. 职责:
压力蒸汽灭菌操作人员对本标准的实施负责。
5. 程序:
5.1. 本指示卡用于下排气式压力蒸汽灭菌器灭菌效果的监测，指示卡上的指示色块在灭菌加热时由淡黄色变为黑色，随颜色变化的深浅可判断灭菌处理是否达到要求。
5.2. 使用时，将指示卡放入欲灭菌物品包内中央部位。灭菌后，指示色块深度达到或超过指示卡上的标准色块，表示所达到的温度和持续时间已满足灭菌所需条件(121?30分practices. The development of various undertakings, requires each party to pay and hard work. No free ride thing under the Sun, if you cannot play a vanguard and exemplary role of party members is unworthy party members. Both staff members and no staff members, both vigorous and resolute working style, good at policy advocacy, and to perform good decisions of party committees and Governments, determined to complete work assigned by the superior party. Third, focus on improving service, the ability
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钟)若浅于标准色块，表示作用的温度和持续时间未达灭菌要求。
5.3. 指示卡受水湿可影响变色准确性，故灭菌时尽量避免与金属、玻璃等表面易有凝
集水或其他潮湿物品接触。
5.4. 本卡应保存在室内干燥、阴凉处。
6. 附:化学指示卡测试灭菌效果记录ZL0082 00。
practices. The development of various undertakings, requires each party to pay and hard work. No free ride thing under the Sun, if you cannot play a vanguard and exemplary role of party members is unworthy party members. Both staff members and no staff members, both vigorous and resolute working style, good at policy advocacy, and to perform good decisions of party committees and Governments, determined to complete work assigned by the superior party. Third, focus on improving service, the ability
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