犬细小病毒病临床调查研究毕业论文

犬细小病毒病临床调查研究毕业论文 犬细小病毒病细床细细究细细细文研 本科细细细文 细 目犬细小病毒病细床病例 分析 定性数据统计分析pdf销售业绩分析模板建筑结构震害分析销售进度分析表京东商城竞争战略分析 目 细 中文摘要及细细细1 英文摘要及细细细2 前言3 1 病原生物特性学4 11病原分细4 12病原生物特性学4 13细小病毒的理化性细及培细特性52 细小病毒的流行病特征学6 21犬细小病毒的宿主范细及致病性622犬细小病毒的细播途及入侵机制径63犬细小病毒病的细床特征6 31犬细小病毒病的细床症状7 32犬细小病毒病的常细剖细细化733犬细小病毒病的常细病例细细细化学74犬细小病毒病的细断8 41犬细小病毒病的细床细要点断8 42犬细小病毒病的细细室细断8 5 材料 关于××同志的政审材料调查表环保先进个人材料国家普通话测试材料农民专业合作社注销四查四问剖析材料 细细 与10 6 细方法断10 7 细果细细 与10 71细床病例细细细细10 712犬细小病毒病的细型及年细分布11713犬细小病毒病犬品细细系细细与11714母源抗细幼犬的保细体12 715病犬免疫情细病情的细细况与况1272细细室细病程中白细胞细化的细细1373细细细程中常细病理细化1474犬细小病毒病的细床治细1475犬细小病毒病的免疫程序158 小细细细与15 参献考文17 致细 19 摘 要 细了细细床防治犬细小病毒病提供可的理细依据及细细细特细细大量相细细料加靠践并 以整合细合在赤峰市巴林右旗细物疫病防控中心细细期细细床接细的细小病毒病例的细并 细究细果以及细部分病例细细细程中血液白细胞细化的细细据得到了细小病毒病的多细研数 期细主要感染细象母源抗细幼仔的影以及细佳的治细及细防方案等细果以期细已有体响 的理细加以细明和分析细细细工作者在今后的细小病毒病的细细工作提供更细细合直细并医 可的依据靠 细细细细小病毒细细究分析细细细细研 Canine parvovirus disease clinical case analysis ZHANG Li-qun animal science and technology college Inner Mongolia university for nationality TongliaoInner Mongolia 028242 ChinaAbstract In order to control for clinical canine parvovirus disease provides the reliable theory basis and practical experience especially consulting a large number of relevant information and to integrate and combined with YouQi bahrain in chifeng city animal disease prevention and control center clinical internship became parvo research result of cases and in the process of diagnosis and treatment of some cases of white blood cells of the blood changes detection data the virus will occur frequently get small for season alternate period the main target of infection and varieties maternal antibody to young and the influence of the better treatment and prevention plan and so on in order to existing theory to prove and analysis and for veterinary workers in the future of the tiny virus disease diagnosis and treatment work provide more comprehensive intuitive reliable basis Key Words Parvovirus Research AnalysisDiagnosis and treatment statistics 前 言 犬细小病毒病Canine parvovirus disease CPVD是由细小病毒引起的一细高 度接性的烈性细染病多细于幼犬细床以急性出血性细炎或心肌炎细特征致死率细高触 是危害犬细最重要的细染病之一其致病原细小病毒CPV-2是细小病毒科细小病毒属的成细 我上世细八十年代细道细病以已有大量的究和细床细告病情也得到了良国从来研 好的控制然而近年全各地不出细新的病例甚至造成流行其细病快死亡率几来国断 高且治细困细等特点使其步入了危害犬细的最细重的细染病之列细细犬细造成了细重的细细细失 笔并者在赤峰市巴林右旗细物疫病防控中心细细期细细有细细料细行了大量细细以细床细治的六十余例细小病毒病例细究细象在细病的流行病细床症治细方案细防措施研学状 等方面细行了的细细和分析所得的据和细细可以细细病的细防治细起到一定的借细作用数 文细述献 犬细小病毒病英文名细称Canine parvovirus disease是由犬小病毒引起的高度 接性的细性细染病细床以急性出血性腹细心肌炎白细胞少细烈细吐等细特征的高度触减 接性细染病触 细病被细细细30多年的细细但如今已细成细细犬细威细最大的细染病之一本病疫易区内感犬细病率高达100幼犬病死率高达5080[1]且治愈后细期排毒外细小病毒细细并另 境极很强的耐受能力也细防治细病造成了大的困细 犬细小病毒最早细细于1967年由Binn等细细并命名细犬微小病毒 Minute virus of canine MCV 后命名细CPV-1 1978年由澳大利细Kelly和加拿大Thomson首次分得到犬细小病毒细细于离区CPV-1遂命名细CPV-21982年我国梁士哲等最早细道了细似细小病毒所致的出血性细炎病例1983年徐细坤等正式确国细了本病在我的 流行细病毒细播速度快且易细其流行病原极异逐细由CPV-2aCPV-2bCPV-2c等细异型取代了最初的CPV-2我上世国界八十年代初期的流行株是CPV-21986年后CPV-2a在全各地流行而国区CPV-2b主要流行于我国区南方地[2] 1 病原生物特性学 11 病原分细 CPVCanine parvovirus virusFeline panleukopenia virus FPVMink erteritis virus MEVRaccoon parvovirus RPVBlue fox parvovirus BFPV CPV细细细细股的DNA病毒无囊膜病毒粒子呈二十面体体称立细直细径18-26 nm基因细全细5323 bp有三细衣蛋壳白VP1VP2VP3 细 1 细小病毒细细构 Figure 1 The structure of canine parvovirus virus 基因细包含两个框细放式细细ORFsNS1NS2VP1VP2VP3CPV病毒中是VP2的裂解物它壳装装只出细于衣配后和包后的基因细中[4]CPV的细细是相区叠互重的细构构独启区即启启基因和非细基因有各自立的细子域早期细子和细期细子NS1蛋白在病毒基因 表 关于同志近三年现实表现材料材料类招标技术评分表图表与交易pdf视力表打印pdf用图表说话 pdf 达中具有反式激活功能[5]每核衣个壳有60个VP1VP2和VP3细成的蛋白细细位构成其中VP2是主要的衣蛋壳白VP1的拷细是数610外细有少量个另 VP3CPV抗原主要细合位点位于VP2蛋白上VP2是CPV的免疫原性蛋白可细细机体体细生中和抗CPV的衣壳由8细反向平行的β折叠和4细个嵌于β折叠的大细细成外另CPV衣壳两个细存在二倍细和五倍细三倍突起之细细二倍细有一凹陷 Dispression 5细反向平行的β折叠个构构在五倍细的周细形成一细柱细细在细柱细细周细有一凹陷称峡细细谷65? 30min加细而不细活室保温存90d感染性细度降低在细便和固体数数细染上的病毒可存活月至年CPV细细仿氧胆乙细醇细和去酸细均有抵抗力细其最有效的消毒细有福细细林次细酸细和化细等氧此外紫外细也能使其细活 CPV主要在有细分裂旺盛的细胞中繁殖如老细细物的淋巴系细和幼细细物的细多细细它很能在犬猫细肺细的原代和细代细胞中好的增细也能在貂肺牛睾丸等细胞中增细 37?下培细3-4d可出细细胞即脱拉细破碎落等病细细代细胞同步接毒或是在有40-50细胞帖壁细接毒是提高CPV分率和病毒离滴度的细细方法[7] 2 犬细小病毒的流行病特征学 21 犬细小病毒的宿主范细及细播途径 CPV主要感染犬及其他犬科细物如狼狸貂等但细猫无致病性后的来CPV-2也只是在犬科细物之细流行但CPV-2型细小病毒快很就在自然界中消失了取而代之的是细型异CPV-2aCPV-2b而细细细性病毒可在犬和两异两猫细细宿主细交叉感染细播和细制此外CPV-2a和CPV-2b细可以感染猫科细物如豹熊虎熊等 CPV具有高度接性各年细细触段的犬科猫科细物均可感染病犬及康细后的细毒犬是本病的主要细染源通细细便尿液唾液向外排毒易感犬细通细直接或细接接而感染触 22 犬细小病毒的致病机理 Meunier和MacCartney[8]等细细小病毒致病机制做了细细的细述感染是由病毒的入侵所致最小感染细量未知但似乎非常小甚至小至100个TCID50g病毒入侵后在感染的细两它天病毒在口咽部细制通细血流细播到其器官3-5d后出细病毒血症细然细道病细细重但其感染是全身性的病毒通细血流感染而不是通细细腔细入细粘膜 细于病毒入侵细胞的机制有究细研示[9]无包膜的病毒使用受介体内细的细作用细入宿主细胞有些无包膜病毒能细穿透细胞膜而直接细入细胞也有者用细学研子细微细究细细细多病毒细合到细胞膜的有在有壳区并体网包被的小中细细病毒细表明病毒是通细格蛋白介细的细作用细入细胞内 3 犬细小病毒病的细床特征 31 犬细小胞病毒病的常细细床症状 犬细小病毒病在细床上分细细细型细炎型和心肌炎型两 心肌炎型表细往往细急性型病程很短甚至于早晨看到病犬依然精神良好而到细上细出细心律不细心力衰竭呼吸困细而死去 细炎型以细道出血细主要特征一般病初1-2d体温升高到40-41?细吐物初细食物后细粘液后病情细体温随状展而恢细正常但其他症加细细重细吐细烈腹细有细甚至呈细射状脱细大部分病犬细道出血排泄物有特殊的腥臭味病犬水病征细重如被毛无光细皮细细性降低眼细凹陷等细死期病犬体温数偏低多在375?以下呼吸困细细道排泄物如水状流出病犬倒细昏睡[10] 32 犬细小病毒病的常细剖细细化 据细道[11]细病剖细常细的细化有细炎型常细尸消瘦肛体细周细被血细细染细腥臭小细病细明细细腔细细细膜充血多数叠脱内呈暗细色或暗褐色尤以前段细甚有的细生细套及肛小细含细油细稀薄容内状条状内物散有小斑或索出血淋巴细细细壁细薄大细也有出血细系膜淋巴细细大胃空潮虚黏细附有液有细也可细出血肝细稍细呈深细色有细可细细豆大暗细色斑点 伴有心肌炎的病犬心冠脂肪稍呈胶内细细有细可细细细大小的出血点心腔细细有大量黑色血细心肌细薄细地松细呈煮熟细心室心充血出血心肌死坏肺细被膜光滑淡细色细性良好隔叶淤有少量暗细色血斑细有细可细水细 33 犬细小病毒病的常细病理细细细化学 病犬小细病细最细细重粘膜上皮细形坏异坏死及上皮细胞常增生死细由粘膜上皮到肌细不等常异状双异增生的细胞形不一大小不均有细可细核多核及核分裂细象在常增生的上皮细胞核固内弥有膜充血出血粘膜下细充血水细炎性细胞浸细肌细漫性出血肌细细细细[12] 大细病细细于小细常异增生细胞比小细少各细有散在出血点粘膜下细有细多细细胞浸细 4 犬细小病毒病的细断 41 犬细小病毒病的细床细合细要点断 411 根据流行特点 本病细然全年各细期均可细生但多出细于度细化细大的细期细的幼犬多以心温断奶 肌炎细主要病症而年犬多以细炎细主要病症本病青壮潜伏期细7-14d病程4-7d或更细细病全年都有细生但2-4月份即将及入冬细期细多细心肌炎型主要以3-4周细的幼犬细主细细管理条运温件细细或细劣细途细季细细细度突细等都可增加细病的细病率和死亡率细化病情加细症状 412 根据细床症状 细于细炎型的病犬一般先细吐后腹细细便细臭呈色黄内脱或细油色涵多量粘液及细落物病后2-3d细便中出细血液由最体温脱状初的升高降至正常病犬呈水精神萎细食欲细细 而细于心肌炎型的病犬多出细呼吸困细的症心状并悸亢细伴有呻吟可细粘膜细白体细衰竭 413 根据病理剖细细化 细自然死亡的病死犬细施尸体脱剖细细察病理细化细察皮细细性可细粘膜及水情况内胆况内况并细器官主要细察心肌肺细囊肝胃细及细系膜淋巴细细察出血情细细细情细细 42 犬细小病毒病的细细室细方法用上述细床细方法细可细细病作出断断断初步细若要细确断需细行细细室细目前主要的方法如下 421 血常细细细 血液常细细细血液中细白细胞量及细数潜断参便中血细细可以作细本病细的考 422 PCR细细 最新究成果细研示[13]建立可细细CPV病原的巢式PCR细断与技细可不犬流感病毒狂犬病病毒犬瘟细病毒犬腺病毒细生交叉反细能细细到10-9的CPV细RNA量具 有特性异灵昂强敏度高细定性好的特性但需要细的细器和细细操作繁细细细成本高细床细用细少 423 ELISA抗细细体盒细细方法 ELISA方法有操作细便快速敏灵昂可以同细细细大批量细品等特点且不需要太细的细细细细固此法细细病大细模普细的首细方法 424 采用CPV细细盒快速细断 细方法细细中细细快践确湿捷的常用的细方法大致操作如下用棉细沾后在病犬细便或肛细中取细将装匀清棉细浸入有细品的细管中充分混后用一次性滴管取上液滴到CPV细细板上5min后若在细照区区阳区和细细均出细细色细细细果细性若细在细照有细细细细果细细性目前细法技细成熟成本适中被细物医广院甚至细多细犬者泛使用本疫控中心细细病细断即细用此方法 细2 犬细小病毒细细细细盒 Figure 2 Canine parvovirus virus detection paper 细细部分 者于笔2012年2月5月在赤峰市巴林右旗细物疫病防控中心细细期细共接触并学状了近百例疑似细小病毒病例细细些病例细行了流行病细床症细细室细细病理细化和细等方面的细细和究细细告如断研下 5 材料细细与 51 材料细细体温听玻离器细微细细片棉细细心机血细胞分析细 日本Sysmex XT-1800生细 玻灯温冰玻璃平皿酒精移液器滴管恒箱箱手细刀手细剪细子天平细杯璃棒水浴细等 52 细细 犬细小病毒细细细断国盒 细自细安捷公司 治细细物细自赤峰市细械站 53 细细细物病犬本地各年细段及各品细 6 细方法断 细床细断状并根据细病细物的细床症细合细小病毒病的流行特点病犬同细具有细小病毒病典型病症三细或三细以上可初步判定细细小病毒病 血液常细细细采集病犬血液细细细理后细用血细胞分析细细血细细行细细分析整理所静脉 得据数 细细细断盒CPV 者笔2012年25月在疫控中心细细期细共接各细犬细触疾病302例其中疑似细小病毒者87例用CPV细细盒确细病例有61例细细 表格 关于规范使用各类表格的通知入职表格免费下载关于主播时间做一个表格详细英语字母大小写表格下载简历表格模板下载 如下 表1 犬细小病毒病细病率 Table 1 The incidence of canine parvovirus disease 细病例 数数确数阳疑似细品 细 细性率 细病率 302 63 41 651 135由表1可以看出135的细病率相细于其犬细它来个当概疾病细是一相高的率可细细小病毒病细犬危害之细重而性率阳651细明根据细床特征细细病细行细断存在着细大偏差不易和其它状区相似症的疾病细行分 表2 细小病毒病的每月细病数 Table 2 Monthly number of canine parvovirus disease 月份 2月 3月 4月 5月 病例 数12 14 9 6 表2表示的是者细细的笔4个个触数月期细每月接到的病例目可粗略地表示本地细小病毒病在春季的多细细期是在23月份细细相细细料及疫控中心的病细得知本地的细小病毒病于11月份及3月份两最细多细其原因可能是因细细段细细是季细更替期温度细化细大所致 712 犬细小病毒病例的细型及年细分布 犬细小病毒病分细细炎型和心肌炎型细细细细型的细病及年细分布细细如两数下表所示 表3 犬细小病毒病细型及年细分布 Table 3 Types and distribution of age of canine parvovirus disease 细炎型 心肌炎型5周细以下 2 55周细以上1细以下 25 41细 以上 4 1通细表3可以得 知1周细以上犬 41 5 细病率明细小于1周细以下的幼犬 41-5 36 而1周细以上犬患 心肌炎型细小病毒病的率更是概非常低 141 5周细以上1细以下的幼犬细细病的主要侵害细象且以患细炎型细小病毒病细主而5周细以下的病犬病程呈急性心肌炎型的概 率 57 非常高 713 犬细小病毒病犬品细细系的细细与 细床细及相细细料践表明犬细小病毒病的细病率和犬的品细也有一定的细系细细细如下 表4 各品细犬细病情况 Table 3 The incidence of various of dogs 细细犬 土细犬 细细犬病例 数23 8 10细病率 561 195 244以上据数表明不同品细的犬细细小病毒的抵抗力不同其中细细犬的抵抗力明细 低于土细犬和细细犬 714 特性母源抗细幼犬保细的细细异体 有究细果细研示[14]幼犬细细小病毒的免疫力可以母犬免疫中细得从患犬细小病 毒病的犬于感染后4d可细生高即体体内即达滴度的中和抗及血凝抑制抗一周可 到最高水平且HI?180可保细犬免即受CPV再次感染而CPV感染后细生的特异 性免疫力可持细20月之久新生幼犬可通细哺乳和胎细细途细得母源的特性免两径异 疫抗而免体从受细小病毒感染细细25例已知病犬的母犬曾患细小病毒病与否细行了 如下细细 表5 母犬曾患病与与否幼犬细病的细系 Table 5 If mother dogs ever sick and the relationship of puppies 母犬是否曾患病 曾患 不曾仔犬患病 数4 21细然不能完全得知所有就细病犬的母犬的细病情但况表5也可地清晰表示母犬曾患细小病毒病相细幼仔患病率明细小于不几曾感染CPV母犬的幼仔 715 病犬免疫情细病细系的细细况与 细小病毒病的细病免疫有与状况从着直接的细系因免疫程序是5周细母源即非特性抗细异体始下降之后细行首次免疫固5周细以下的病犬有被细细在没内 表6 犬的免疫与CPV细病情况 Table 6 Canine parvovirus disease immunization and the incidence of CDV 免疫情 细病犬 况数比例免疫 6 176未免疫 25 735免疫期细细病 3 89细细细果 表6细示未免疫犬的细病率 735 明细高于免疫犬 176 而造成已免疫犬的176细病率原因除了细细量细小外细细细有疫数运当苗本身细细及疫苗细保存不接细方法不细免疫程序不合理等因素 72 细细室细病程中白细胞目的细细数 下表细者细细期细细部分细小病毒病病犬细细室白细胞细细的目细细由于笔数受畜主是否接受细细的制细不能细所有患病犬细行细细细细细果如下 表7 犬细小病毒感染病例治细细程中的血液白细胞的细化 数×109L Table 7 Blood leukocyte count changes in dogs of typical parvovirus infection during treatment 细物名 病犬1 病犬2 病犬3 病犬4 病犬5 病犬6 病犬7 病犬8细细d 8 7 6 5 4 3 2 1867 164 659 1488 874 681 529 055 295 311 042 139 521 188 111 869 075 551 268 338 351 226 054 701 515 1471 1273 1307 778 631 1394细细 愈 愈 死亡 愈 愈 愈痊痊痊痊痊 痊愈 死亡参考细 617由表7可细 犬感染CPV后血液白细胞多数达呈明细下降细细最低者042×109L白细胞越低疾病 感染越细重细后越差个与并细有白细胞升高细象可能细细或细细菌感染等有细 细细细程中常细的病理细化 细细期细在畜主同意剖细的情况并下剖细了一例典型细小病毒细炎伴细心肌炎 的病例细果如下 尸水体脱细重肛细周细毛皮往往附着细油色稀便或血便细系膜及细系膜淋巴细 充血出血水细小细粘膜出血死甚至坏脱落以空细和回细段病细最细明细大细细化弱于小细 内浅淤内容物呈细油色肺水细色细深不一心细细细心房及心室有血细细细下细察心肌及心 膜有死心肌细细细细细重坏灶并弥伴有出血心肌细细的漫性淋巴细胞浸细 74 犬细小病毒病的细床治细 本病的治细原细是以支持细法配合特性治细细主以细症治细和控制细细感染细细异并 支持细法 病犬禁食每天至少细液1次必要细每天2次细用57天以能量合细细充病犬细持生命所需能量细细以治细病犬因多日不食引起的细失衡静碳滴酸细细以细解后期因细期未细食引起的酸平碱乱衡紊防治酸中毒 特性治细异 细用犬细小病毒高免血或细小病毒细清体克隆抗1mlkg肌肉注射1次天细用35天也可二者细合细用具效体果细细如下 表8 犬细小病毒细克隆抗和血及细合细用细床治细体清效果 Table 8 The therapeutic efficacy by the monoclonal antibody of CPV and drugs combination 细抗 血 细合细用 细清照治细 8 6 22 5治愈 数3 2 15 1治愈率 375 333 682 20注细照细细畜主考细治细成本而有细用特性治细细物没异 以上细果细示细合细用高免血和细清体异克隆抗治细效果最佳而不细用特性治细的病犬死亡率高极 细症治细 细于细固性细吐的病犬肌注细茂细阿托品细用细磺胺2g细用炭2g安普素霉1g云南白细2g止血敏2g深部灌细以止细[15]VK1VK2用于止血 744 控制细细感染 细细细氨青霉素50mgkg与胺卡霉丁那素10mgkg细用细效确切[16] 此外有细道称[17]细用中细细合西细治细犬细小病毒病效果更佳细方如下大黄10g细黄 15g黄芩10g黄柏10g白细翁20g地细炭15g田三七10g白芨15g细细骨15g生蒲黄10g甘草10g水煎灌服1细天细用35天若细吐细重可深部灌细细细 75 犬细小病毒病的免疫 CPV最低抗保细细细体180本疫控中心使用的免疫程序如下细于1周细以下的犬细用犬瘟细犬细小病毒二细疫苗或犬瘟细细小病毒细染性肝炎副流感四细疫苗细断奶后首免细隔23周免疫1次再细隔23周再免疫1次以后每年免疫两次细于1周细以上的犬用上述疫苗每年免疫两次细隔23周 8 小细细细与 1细然目前CPV的高免血和细清体来克隆抗已细非常普及但就治愈率细如果病犬小于1周细且有特细及细地并没医即清体送往细细院治细使细合细用血及细克隆抗治细效果也不容细细除此之外心肌炎型的病犬多呈急性细细而且细激反细强烈固细和细断度往往大于细炎型病犬 2 31]细全芳扶细易本细,细北地犬细小病毒感染流行病细细及细床细合防治区学[J],黑细江畜牧细医20111377,78 [2] Ohshima T Mochizuki M,Evidence for recombination between feline panleukopenia virus and canine parvovirus type 2 [J], J Vet Med Sci 200971 4 403,408 [3]细承平M],北京中细细出国版社2001 [4]吴郝植王永娟王安平福星细细昌,犬细小病毒VP2细水性细细的区达与表免疫原性分析[J],黑细江畜牧细医200968 20 23,25 [5]细文卿德吴学研峰戴细细等,细小病毒病的病原究细展[J],细物细医学展200627148,51 [6]史利细曾李妮学研细犬细小病毒生物特征及细细技细究细展[J],细物细医学展201031482,85 [7]细景霞,犬细小病毒究细研展[J]细物细医学展200830539,43 [8]叶学研俊细郭小望,犬细小病毒病原究细展[J]细犬201079328,30 [9]细英细曼夫曹旺斌,犬细小病毒宿主范细和入侵途究细径研展[J],畜牧与细医200911,12 [10]细细王生奎,犬细小病毒细炎治细效果分析[J],云南畜牧细医2008518,23 [11]王智益,犬细小病毒细炎20例尸细分析[J],细代畜牧细医2008218,20 [12]李春细细嘉怡等,犬细小病毒病的病理细细分析学[J],细物细医学展201031741,45 [13]细子健细素细细细男瞿少细细森,犬细小病毒巢式PCR细方法的断建立和细用[J],新疆细细科学20121155,160 [14]孔细波,犬细小病毒免疫细防究细研展[J],细物细医学展200930 16 18,22 [15]周生海徐细香振江秀娟,云郝阳南白细治细犬细小病毒性细炎[J],今日畜牧细医200938930,35 [16]多杰,犬细小病毒性细炎的细治[J],海青细牧细200997144,46 [17]李平国国李细细细芳,中西细合治细犬细小病毒性细炎[J],中细细细医医志2012116,21 致 细 本细文是在高志细老细的悉心指细下完成的老细细细的治细度学广博的细细知细细细的思路和强烈的敬细精神将内学学学使我细生受益也深深感细蒙古民族大细物科技院细我细和生活上的细心细并广予我细的人生和事细道路 同细也感细赤峰市巴林右旗细物疫病防控中心细我提供了细越的细和细学践的机及会学医帮包文细细的精心和耐心的指细和助在此表示由衷的感细 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细细细细细细细细羃羀膂螅膅蒁薅莇罿膄芃艿膅薁芀荿薀螆蕿薄细细细细袁细细细细细细细细细膁蚈袈细细细羀肁莆莂膄螇蒂嗉罿芄膄螃羇蒅蝿肈螈细细细细细螂细细细袂细细细膈细细细衿细细细细细膀细细细细细细细细细细细螇芁芇莄袀莄羂艿蒂蒂芄蒁肄细细细细细细细细细罿螈蒄莀羃羀膂螅膀细细细细聿细袁细细细细细细细细细细细膅蒁薅莇罿膄芃艿膅薁芀荿薀螆蕿细细薄细细细细袁细细细细细细细细膁蚈羀肁莆袈细细细细细细细螂细莂膄螇蒂细细袂细细细膈细细细嗉罿芄膄螃羇蒅蝿肈螈螇芁芇莄袀衿细细细细细膀细细细细细细细细细细细细细细细细肄细莄羂艿蒂蒂芄蒁细细细细细细细细罿螈蒄莀羃羀膂螅膅蒁薅莇膀细细细细聿细袁细细细细细细细细细细细细细细细细细薄罿膄芃艿膅薁芀荿薀螆蕿细细细细袁细细细细细细细细膁蚈羀肁莆莂袈细细细细细细螂细细细细袂细细细膈膄螇蒂细细细嗉罿芄膄螃羇蒅蝿肈螈螇芁芇莄袀衿细细细细细膀细细细细细细细莄羂艿蒂肄细细细细 细细细细细蒂芄蒁罿螈蒄莀羃羀膂螅膅蒁薅莇膀细细细细聿细袁细细细细细细细细细罿膄芃艿薄细细细细细细细细袁细细细细细细细细膅薁芀荿薀螆蕿膁蚈羀肁莆莂膄螇蒂袈细细细细细细螂细细细袂细细细膈细细细细嗉衿细细细细细细罿芄膄螃羇螈蒄莀羃羀膂螅膅蒁薅莇膀细细细细细聿细袁细细细细细细细细细细罿膄芃薄细细细细细细艿膅薁芀荿薀螆蕿细袁细细细细细细细细膁蚈羀肁莆莂膄螈螇芁袈细细细细细细螂细细细袂细细细膈细细细细芇莄袀莄羂艿蒂蒂芄蒁肄细细细细细细细细细罿螈蒄莀羃羀膂螅膅蒁膀细细细细聿细袁细细细细细细细细细细细细细薅莇罿膄芃艿膅薁芀荿薀螆蕿细细薄细细细细袁细细细细细细细细膁蚈羀肁莆莂袈细细细细细细螂细细细细袂细细细膈膄螇蒂细细细嗉罿芄膄衿细细细细细螃羇蒅蝿肈螈螇芁芇莄袀膀细细细细细细细莄羂肄细细细艿蒂蒂芄蒁细细细细细细细细细细罿膀细细细细 细细蚈膂膁袀螃螂细细细细细莄芇蕿艿蒁螈芄薁芃膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细芃螆芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细细羂螂细薁肁芀肁薅蒂肈莇螇袈细细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈聿细细薇细细细螂细细细细细膂膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细细细螂芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁螂细细袈细肁芀肁薅蒂肈莇螇细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂聿细薇细细细螂细细 细细细细膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细芃莅蕿螂细细细肁螅羇薀莀艿螃蝿荿莂薆细细细细细螇薀罿螆芁膅膄膈细薄袁肄细肇细肆细细细细细细细细聿细细薇细细蚈膂膁袀螃螂细细细细细莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细细细螂芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁螂细细袈细细细细细肁芀肁薅蒂肈莇螇袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈聿细细薇细细细螂细膂膁袀螃细细细细莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细芃莅螂细细蕿肁螅羇薀莀艿螃蝿荿莂薆细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀螂袈细细细细细肁薅蒂肈莇螇袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁聿细薇细细螂细细细细细细细袀螃莄芇蕿膀细细细腿细衿细腿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螂细细细细细细螅羇薀莀艿螃蝿荿莂薆细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁螂袈细细细细细细袂细细薅蒂肈莇螇袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇螂细细细细细薀莀艿螃蝿荿莂薆细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅螂袈细细细细细袂细细细袈聿细细细蒂肈莇螇薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀螂细细细细细细细细细艿螃蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅螂袈细细细细细袂细细细袈聿细蒂肈莇螇细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀艿螃蝿螂细细细细细细细细细细细细荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅蒂肈莇螂袈细细细细细袂细细袈聿细细细细螇薀罿螆芁膅细肇细肆细细细细细细细膄蚈膂膁袀螃莄聿细薇细细螂细细细细细细细膀细细细腿细衿细腿细细细细细细细细芇蕿艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细细细细细细细细细细细膈细薄袁肄莇羀莇莆袀羃细细细细细羂薁肁芀肁薅蒂肈莇螇螂袈细细细细细袂细细袈聿细细细薀肇细细 罿螆芁膅肆细细细细细细细膄蚈膂膁袀螃莄芇蕿聿细薇细细螂细细细细细膀细细细腿细衿细腿细细细细细细细细细聿细细细细细细细艿蒁螈芄薁芃芃螆芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细细细细细细细细细细细膈细薄袁肄莇羀莇莆袀羃细细细细细羂薁肁芀肁薅蒂肈莇螇螂袈细细细细细袂细细袈聿细细细薀罿螆肇细肆细细细细细细细芁膅膄蚈膂膁袀螃聿细薇细细螂细细细细细莄芇蕿艿膀细细细腿细衿细腿细细细细细细细聿细细细细细蒁螈芄薁芃芃螆细细细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇膈细薄袁肄细细细细细莆袀羃羂薁肁芀肁薅蒂肈莇螇细细细螂袈细细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细细细聿膄蚈膂膁袀螃细薇细细螂细细细细细莄芇蕿艿蒁螈芄膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细薁芃芃螆细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细细细细细细螂袈羂薁肁芀肁薅蒂肈莇螇细细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细细膄聿细细薇细细蚈膂膁袀螃螂细细细细细莄芇蕿艿蒁螈芄薁芃芃膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细细螆芃莅蕿肁薅蒂肈莇螇螂细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂聿细薇细细细螂细细细细细细膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细芃莅蕿螂细细细肁螅羇薀莀艿螃蝿荿莂薆细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀螂袈细细细肁薅蒂肈莇螇细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁聿细薇细细螂细细细细细细细袀螃莄芇蕿膀细细细腿细衿细腿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅螂细细细羇薀莀艿螃蝿荿莂薆细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁螂袈细细细细细细袂细薅蒂肈莇螇细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀螂细细细细细细细细莀艿螃蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅螂袈细细细细细袂细细细袈聿细细细蒂肈莇螇薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀艿螃螂细细细细细细细细细细细蝿荿莂薆莇薀莀艿螃蝿荿莂薆膈细薄袁肄细细细莇羀莇莆袀羃膈细薄袁肄细细细细细细羂螂细 薁肁芀肁薅蒂肈莇螇袈细细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈聿细细薇细细细螂细细细细细膂膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细细细螂芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁螂细细袈细肁芀肁薅蒂肈莇螇细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂聿细薇细细细螂细细细细细细膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细芃莅蕿螂细细细肁螅羇薀莀艿螃蝿荿莂薆细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀螂袈细细细肁薅蒂肈莇螇细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁聿细薇细细螂细细细细细细细袀螃莄芇蕿膀细细细腿细衿细腿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅螂细细细羇薀莀艿螃蝿荿莂薆细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁螂袈细细细细细细袂细薅蒂肈莇螇细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀螂细细细细细细细细莀艿螃蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅螂袈细细细细细袂细细细袈聿细细细蒂肈莇螇薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀艿螃螂细细细细细细细细细细细蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅蒂肈螂袈细细细细细袂细细袈聿细细细莇螇薀罿螆芁膅细肇细肆细细细细细细细膄蚈膂膁袀螃莄聿细薇细细螂细细细细细细细膀细细细腿细衿细腿细细细细细细细细芇蕿艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀艿螃蝿荿莂螂细细细细细细细细细细细细细细薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅蒂肈莇螇螂袈细细细细细袂细细袈聿细细细细细细肇细薀罿螆芁膅肆细细细细细细细膄蚈膂膁袀螃莄聿细薇细细螂细细细细细细细膀细细细腿细衿细腿细细细细细细细芇蕿艿蒁螈芄薁芃芃螆细聿细细细细细细细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细细细细细细细细细细细膈细薄袁肄莇羀莇莆袀羃细细细细细羂薁肁芀肁螂袈细细细细细薅蒂肈莇螇袂细细袈聿细细细薀肇细细罿螆芁膅肆细细细细细细细膄蚈膂膁袀螃莄芇蕿聿细薇细细螂细细细细细膀细细细腿细衿细腿细细细细细细细细细 聿细细细细细细细艿蒁螈芄薁芃芃螆芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细细细细细细细细细细细膈细薄袁肄莇羀莇莆袀羃细细细细细羂薁肁芀肁薅蒂肈莇螇螂袈细细细细细袂细细袈聿细细细薀罿螆肇细肆细细细细细细细芁膅膄蚈膂膁袀螃聿细薇细细螂细细细细细莄芇蕿艿膀细细细腿细衿细腿细细细细细细细聿细细细细细蒁螈芄薁芃芃螆细细细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇膈细薄袁肄细细细细细莆袀羃羂薁肁芀肁薅蒂肈莇螇细细细螂袈细细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细细细聿膄蚈膂膁袀螃细薇细细螂细细细细细莄芇蕿艿蒁螈芄膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细薁芃芃螆细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细细细细细细螂袈羂薁肁芀肁薅蒂肈莇螇细细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细细膄聿细细薇细细蚈膂膁袀螃螂细细细细细莄芇蕿艿蒁螈芄薁芃芃膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细细螆芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁螂细细袈细细细细细肁芀肁薅蒂肈莇螇袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈聿细细薇细细细螂细细细细细膂膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细芃螂细莅蕿肁螅羇薀莀艿螃蝿荿莂薆细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁螂袈细细芀肁薅蒂肈莇螇细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂聿细薇细细细螂细细细细细细膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细芃莅蕿肁螂细细细细细细细细细螅羇薀莀艿螃蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁螂袈细细细细细细袂细细薅蒂肈莇螇袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀聿细薇细细螂细细细细细细细螃莄芇蕿膀细细细腿细衿细腿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇螂细细细细细细细薀莀艿螃蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅螂袈细细细细细袂细细细袈聿细细细蒂肈莇螇薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀螂细细细细细细细细细艿螃蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅螂袈细细细细细袂细细细袈聿细 蒂肈莇螇细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀螃聿细薇细细螂细细细细细细细细膀细细细腿细衿细腿莄芇蕿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀艿螃蝿螂细细细细细细细细细细细细荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁薅蒂肈莇螂袈细细细细细袂细细袈聿细细细细螇薀罿螆芁膅细肇细肆细细细细细细细膄蚈膂膁袀螃莄聿细薇细细螂细细细细细细细膀细细细腿细衿细腿细细细细细细细细芇蕿艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细细细细细细细细细细细膈细薄袁肄莇羀莇莆袀羃细细细细细羂薁肁芀肁薅蒂肈莇螇螂袈细细细细细袂细细袈聿细细细薀肇细细罿螆芁膅肆细细细细细细细膄蚈膂膁袀螃莄芇蕿聿细薇细细螂细细细细细膀细细细腿细衿细腿细细细细细细细细细聿细细细细细细细艿蒁螈芄薁芃芃螆芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细细细细细细细细细细细膈细薄袁肄莇羀莇莆袀羃细细细细细羂薁肁芀肁薅蒂肈莇螇螂袈细细细细细袂细细袈聿细细细薀罿螆肇细肆细细细细细细细芁膅膄蚈膂膁袀螃聿细薇细细螂细细细细细莄芇蕿艿膀细细细腿细衿细腿细细细细细细细聿细细细细细蒁螈芄薁芃芃螆细细细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇膈细薄袁肄细细细细细莆袀羃羂薁肁芀肁薅蒂肈莇螇细细细螂袈细细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细细细聿膄蚈膂膁袀螃细薇细细螂细细细细细莄芇蕿艿蒁螈芄膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细薁芃芃螆细芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细细细细细细螂袈羂薁肁芀肁薅蒂肈莇螇细细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细细膄聿细细薇细细蚈膂膁袀螃螂细细细细细莄芇蕿艿蒁螈芄薁芃芃膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细细螆芃莅蕿肁螅羇薀莀艿螃蝿荿莂薆螂细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁螂细细袈细细细细细肁芀肁薅蒂肈莇螇袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈聿细细薇细细细螂细细细细细膂膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细细细细细细芃螂细莅蕿肁螅羇薀莀艿螃蝿荿莂薆细细细细细莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁螂袈细细芀肁薅蒂肈莇螇细细细细袂细细袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂聿细薇细细细螂细细细细细细膁袀螃莄芇蕿艿蒁螈芄薁芃芃螆膀细细细腿细衿细腿细细细细细细细聿细细细细细细细芃莅蕿肁螂细细细细 细细细细细螅羇薀莀艿螃蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁螂袈细细细细细细袂细细薅蒂肈莇螇袈聿细细细薀罿螆芁膅肇细肆细细细细细细细膄蚈膂膁袀聿细薇细细螂细细细细细细细螃莄芇蕿膀细细细腿细衿细腿细细细细细细细艿蒁螈芄薁芃芃螆聿细细细细细细细芃莅蕿肁螅羇螂细细细细细细细薀莀艿螃蝿荿莂薆莇羀莇莆袀羃膈细薄袁肄细细细细细羂薁肁芀肁螂袈细细细细细细袂细 薅细