【doc】几种淡水蚌外套膜蛋白质氨基酸组成及部分元素分析

【doc】几种淡水蚌外套膜蛋白质氨基酸组成及部分元素 分析 定性数据统计分析pdf销售业绩分析模板建筑结构震害分析销售进度分析表京东商城竞争战略分析 几种淡水蚌外套膜蛋白质氨基酸组成及部 分元素分析 1.14No.3 NATURALPRODUCT-RFSEARCHANDDEVETAPMENT Y??????5???M?????????e?e???????- ????Q6Y+??i6i0 ~???Q3Yz?iQ3??i6 Q6?~???.<X?m??????????<B?<?????>?? (?????g???r#????????-??610064) ???5???M?????d<???]6?e??Q????????e?"??x. Y??w?????5??;?M????????;?e????;Yz?i~?<?B <U???3??x1?3??????????#??K??Y????d?F???? ?5????<???Y?????<9??#???x.?P????<X????~????M ??Q0?dY????U?'???????????'??x.????]?<I#O?8<h ???%#?]6????#??????????????g?????/?????P???g ???.?I???J~??????????<?j?????-<I??Q?????Y??? ???r~??}??Q?Q?x.?F??Y??????5??????<???~????? ~q#e?P???K????#D???M????x1????Q0????~z#e[(11 ??QZ??????<?<x????Q????????"???I<]??Q????P?P ???4??????????~?])?%??x1~??ex1~????P???:???i?- ?"???????/?P~????????i?-<m??????x.??QZ?P?????? ?????5?????M?????mY??i???H?????,???~x.?H?o?? ???)???????????5???M?????????e?e????#???Q3Yz ?iQ3???P<I?????????5?????M????????<?????~?Qa ?e??]??-Q3????x. 1?%??<D???? 1.1?%?????? <U???3??(HyriposiscumingiiHea),????Y??? (Cristariaplicateleach)#?????<X??(Lawprotula Leai)??????~(?????.?C~???;?3??????????(An- odonto<?x1xldiavtapacificaHeude)????????<??????? ???5??x. 1.2???? 1.2.1?%???N?3 ~?]~??250--300g?????5???P??<,#???10~??P ~?Y??M?????P#T???PQ????P??<?60p4????<h?E?????? 8h?PY??e<??????-???t?P?A?ex. 1.2.2??????#t??Q)?? Q3QtY?????~?#N???????M??????<?Y??C????<h Q?<?5ml?~????~?200mg????????QJ(K2SO4:('u- S(Y?6:1)?P?8??<R??~M6h??????<I???????PY?~?#D ??????????50ml??????<h??????x.Q3Qt~?<?<R?? ???P?eY??C???w??]???#t?w???P????????????#t??x. 1.2.3?e???????-Q3?? u?Q3Qt??~??????M?????P?e6mol/L?????5???P Y??e???r?e????Q3??<??+???e???????-~?#t??x. u??M????????<h?????e????(essentialamino acid,EAA)x1~_?????e????(semi-essentialamino acid,SEAA)x1???????e????(non-essentialamino acid,NEAA)??#t?????e??????x. u??M?????????e?e???????mY???<??e?e????Q3 ??~]minoAcidScore??AS)????Q3??x.AAS????Y? ?C??<L: AY?5<? ??????Y+??I9??IN????~??????????w????%? 1gVa????<X?h<u?????e????????Y+?? ?K?k???r2002-01-I7]??????r2002-03-07 ???????K???????????/?"Q?????,NO:39970577 <??)???#??<?:(Comspondingauthor) X100 1.2.4???5???M??????Q3Yz?RQ3?? Y?????~??????M?????.???PQ?<?1ml?~?????P Y?Q?<?Y????~????.]??M????Y&Q3?????P?3??????Y? 250ml??????<h????x.~?<?<R?????e~??%#'?KYW ?v??#??W???%#'?KYW?v???+??<U???3??x1?3?????? ????x1?K??Y????M????<h12??Yz?i#t??x. 46?a?K<????H??<D??~?Vol.14No.3 2????<D???? 2.1Y??????5???M???????????e#t<H ?v??t~????P?????????5???M????<h?P?????e#t ??<?<O??#P?P???????d<U???3???P??]<???d????<X ??x.<U???3???M???????????e#t???d????<X???M?? ????????5.3%Ox.?K??Y???#??3???????????M???? ???????e#t??Q0Q3Qt?d????<X???M??????????4.t %O#?2.8%Ox. 2.2???????5???M????????<h?e???????- ??1???????5???M????<h?????e#tIt(g????/100g?:#?) Table1Contentsofproteininaoantellurnoffourkindsoffreshwatermuasel(gprotein/l00gsa mple) ???5??#??? (Smples) <U???3?? (Hymiposis cumingiiHn) ?3?????????? Ancdontav.ariiaaa rati,fcaHeudc) ????Y??? (Gistariaplicata Leach) ????<X?? (Lawprotula #t?w??(g/IDog) (CrnemtaofN(g/100g) ?????e#t??(g/100q) (Cntenrs??pmtein(g/l?og) I-i) 6.6153.5245.1431.246 41.3422.0332.14 ??2???????5???M?????w???????-<D#tIt(g?z????/1008?:#?) Table2Aminoae-idsandtheircontentsintnantellumoffourkindsoffreshwatermussJ(gAA/ 100gsample) ?e????#O?? (Aminoacid) <U???3?? (<?yriposis cammgiiHex) ?3?????????? (Ancdontarzmdiana. ?K??Y??? (Cristi6<?plimta Aaci..("caHeude) ????<X?? (LamQ?~?Gt ?aY??e??(&.v) ???e??(Thr) <[?e??(5r) ?:?e??(GI~? ?6?e??(Gly) <0?e??(Ala) ???e??(Cy.) ???e??(V-1) ?f???e??(Met) ?.<??e??(11e) <??e??(Leu) ???e??(Ty) ??<0?e??(Phe) ???w??(Lys) ??MO(Hi5) ???w??(Arg) ?d?e??(Pro) #T??(.ml) 1a="h) 4.50 1.97 1.43 4.22 5.07 3.78 0.49 2.39 1.28 2.33 3.99 1.82 2.07 2.69 0.72 3.92 3.03 457n 2.71 1.12 0.92 3.46 2.13 1.69 0.33 1.30 0.61 1.29 1.95 0.90 1.14 1.39 0.40 1.71 1.18 24.23 2.39 1.51 0.84 1oo 3.73 3.82 0.45 2.10 1.02 2.11 3.30 1.39 1.66 2.36 0.50 3.15 2.70 34.03 l-p 1.01 0.33 0.18 0.82 0.45 0.80 0.19 0.71 03() 0.65 0.84 0.35 0.57. 0.36 0.1 0.69 0.65 8.98 ??:???M?????:#??eH~??5???P???e??(Ttp)???5?]?P?&<1x1IJ?,??Y?x. Note:llec-ofthesetnplx.<?hydrolyzedbyHC1,thecnntenteofTrpcouldnotbe ?v??2~????P???????5???M?????>#t?U17???e ????x.Y?<h?????e????7??(Lys,Val,Leu,Ile, Thr,Met,Phe)x1~_?????e????2??(Arg,His)x1???? ???e????8??(Asp,Ser,Glu,Gly,Ala,Cys,Trp, Pro),???5???M???????e????#t??<?<O??#P?P?e?? ???9#t??<????e???g??#??????.?P#t?e?????????? ???d<U???3???M?????PY??p?d?K??Y???x1?3???????? ???P????<X???M????#t?e?????????Fx. 2.3???????5???M????????<hEAA,SEAA, NEAA??#tf ?/????1#???2????Y????5???M????????<h EAA,SEAA,NEAA#t???P????30 Vol.14 ?r<?<?<???<)#???<[?Y?H??#????Y?? No.3 ??3 Tahle3 ???????5???M????????<hEAA,SEAR,NEAR?? #tIt(,.g?w????/g????) EA.A,SEAA.andNEAAcontentsinmantetlumpro- reinoffourkindsoffreshwatermusset(mgAA?8P-- tan) ???5??#???(Samples) Y????3?? (HyriLuiscumin??Hen) ?3?????????? (Anod-wuxrdw-pncificaHeude ?K??Y??? EAASE<?x1NFAA 404112589 96605 (Criswrwplicata ????<X?? (l.aeupmn,la 114508 105572 399 437 477 ??:??Tri?,??Y??P?&#N?????5???M????<hFAA???3Trp??<?x. Notc:B-u-fthema-ilabilityofTrpcontet,Trpwasnotincludo inFAAofallkindsofsamples ?v??3~????P???????5???M????????<h?PEAA ???g?C]??p<I:????<X??>????Y???><U???3??> ?3??????????x.SEAjx1???g?C]??p<I:?K??Y???> <U???3??>????<X??>?3??????????x.NEAA???g ?C]??p<I:?3??????????><U???3??>????<X??> ?K??Y???x.?????e<hEAA#?SEAA??#t???????P?? ???????mY?<?%7<?????x. 2.4???????5???M????????AAS~?????Q3??(21 ?/????f#???2????????Y????????5???M???? ??????AAS?P????4o ?v??4~????P<U???3??x1?3??????????x1?K??Y? ?????M????<h?P??Y+?????e?G#t??#N???????e???? ????Y+????????FAO/WHC)Y??4??iB?3???????ei[ ?????e????#t???AY??P?????P?e<U???3??x1?3?????? ????x1?K??Y??????M????????<h?P<O???e?%QK?g?e?? ??x.??<U???M?????????>??]6?e?????ex.?/?? AAS???g?C~?Qu??:?K??Y????M???????????mY?<? %7><U???3??>?3??????????x.????<X???M?????? ????AAS?X????<?QZ??<U???P]=Y?Lys#t??]<f FAO/WHOY??4??iB?3???????ei[????_ys?AY??PLvs ?d????<X???M???????????%QK?g?e????x.???????? <X???M???????????mY?<?%7??<?<R<U?????M?????? ????]<x. ??4???????5???M???????????e????Q3?? Table4Aminoacidscareinmantellumproteinoffourkindsoffreshwatermusse ?e???? xHY?.inc.acid) FAO/WHO?p?C?v?? (mg/g?????e) FAO/W??)standard' (mg<Zprotein) <U???3?? (Hytiposis croningiiHea) ?9?????????? (Anodonwtcoadiam pxcifinaHeWO ?K??Y??? ((?ftans??in= Lea),) ????<X?? (Lainprotula Leai) 54 109 46 iB 112 42 91 547 156 66 103 73 46 95 47 65 Ile Leu Lys Md+Cys Phe+Tyr 71" Vi #Tf~?(mtal) {:: 59 89 63 43 93 51 ?P 457 131 56 97 65 43 94 48 58 461 40 70 55 35 60 40 50 350 ??:?P1973???##T????Y?????~?<M?{~??r????(FAO/WHO)?##T<E????#?])Y?? 4??iB?3???????e.?????????w?????p?C?, "StandmlofEAAof"prefectprotein"promulgatedQAFAO/WHOin1973 <??????5???M?????eHCxV?5????#w???5?]?,??Y??P?&????AAS???,?3Trp~o ???eY?x. ?5BemusethesampleswerehydrolyzedbyHCI,thecontentsofTrpwerenotincludedinASSo fallkindsofsamples 2.5???????5???M????????<h??#??w????#tf~? ???? Y??U??#????e????~o???a??Y??e????#??:?e?? ??x.?/????1????Y???Y??????5???M???????????? #??e????#t???P????5o ?v??5~????P?e?????????5???M????<h?P??Y+?? ???e?G#t??#??e????????Y+???g?C]??p<I:?3???? ??????>????<X??><U???3??>?K??Y???x.?:?e?? ????Y??U?I??????????#??P?eD-#?L<?<u??????<h?P ~??UL-???U??#?x.?a??Y??e??????Q0?d???L?W?O ???g??#?????<h??<9????#????ex.???????????.?g ??#?~???<9???v??<???#??e?????p?wx.?M???????? ??#D?>~?<??rL???e????x.?????P???????5???M?? ??<??d??~???#??e???????????????"??<+<?x. Vol.14No.3 ??5???????5???M????????<h??#??w??????#t2(mg?w????/g?????e) Table5TastyAAeontentsofmantellumproteininfourkindsoffreshwatermussel(mgAA/gp rotein) ?e???? (Aminoacid) <U???3?? (?Tvripusis cummkiiHea) ?3?????????? (Anal-t,-d.- Q?i6????aHeude) ?K??Y?I (o-tawplicnta Leach) ????<X?? (L'.p.und I-) ?a??Y??e??(Asp) ?:?e??(Glu) #T??(Total) 123 157280 74130 116105 ?? 02 11 1 ??1 ?P ?" 2.6Y??????5?????M????<h??Q3Yz?i#t<H ?v??6~????P<U?????5?????M??????CuYz?i #t???><I?G?+12??Yz?i<h??????x.<U???M????Cu ??#t???g?C<I:<U???3??>?3??????????>?K??Y? ??x.Fe,Mn,#9,Ca,Zn,C:?WYz?i#t?????????)?P Ni,Co,Pb,??x1<?xOx.#t???>???C?PY?<<?C<?~??%#'?K YW?v??#??W???%#'?KYW?v???G???+???????C%7x.?? <????????D?P?M????#t?U<???Q???~?<?]~?G?????? ????Yz?ix. ??6Y??????5?????M??????Q3Yz?i#tf(mg/l00g) Table6Thecontentsofsomeelementsinthreekindsoffreshwatermussel(rng/1008) ????Yz?i#t?? (bntentaofmentalelements) ?K??Y??? Crigariap[icntabeach) <U???3?? (?Ty.ip.iscu-ingiiHea) ?3?????????? Analonm-diarmMcifmHend???? #? Cu Ni ?? Pb zn Y? #e ?? Mo Cr 1.26 11.2 126 <2.33x10-t <2.33~?10"2 <2.33X10' 1.19 21.7 3.84 <1.17X10-3 <2.24x10- <1.67~?to-z ]21 10.8 856 <2.52x10 <2.52x10 <2.52x10 0.629 5.28 4.15 <1.26X10 <3.62x10 <1.77x10 0.84 7.98 736 <2.05x10 <2.05x]() <2.05x10 0.368 4.87 2.86 <1.02x10 <1.66x10 <1.153?W10 <O#P?????5?????M?????????e#t?????e???g?? ?.x.<U???3????]?<I???5?????r<?Y???#???<+?M?P Y??????e#t??????41.34%?PY??U???g????~?Qa?e <?%7x.???M?P????Y??????????eQa?e<?%7??<??3?? ????????#?????<X??x.????<????????e?P???P?)?' EAA?MASS?????PY??mY?<?%7<?????????x.<U???3 ???M???????K?????e#t??????]=FAA#?AAS?>?? ]<?PY??mY?<?%7<???]<x.???M?PFAA?MAAS<?<O?d ??Qu??<??????e?mY?<?%7??}d<??AY?x.????<X???M ????????EAA<I477,AAS<I156?P?>??<?Y?]?<U ?????5???P]=????<X???M????????<h??Lys#t??]< <?FAO/WHCxVY??4??iB?3???????ei[??Lys?AY??P?? ??Lys?d????<X???M???????????%QK?g???e???PY? ?mY?<?%7??Y?]?<U?????5?????M??????????]<x.<? ???????5???M??????????AAS%7Qu???PY??mY?<?%7 ?>??<?????????[[31?n#TQ????????mY?<?%7??<? ?O??????????x. ?e?K??Y???x1<U???3??#??3???????????M???? ????<h?>???e?l???G?+??12??????Yz?i?P???3???5 ???M?????????e%???#??P<O]=~?<?<I<?]~??Y&???? ?e?????P<?~???Y&??Q3?'??#?????Yz?ix. <???<O??Q;?????r<??M?r?9???????ex1?e???? ?????J]?]?<?<?<O?????s?????"????x.<U???3?? ?M????????<h??Asp,Glu,Gly,Ala,Leu,Arg,Pro; ?3???????????M????????<h??Glu;????Y????M?? ??????<h??Gly,Ala,Leu,Arg??Y??UQK?A<?%7?P?? ??<??e??????#t??????????Q3<+<Ux.??QZ?P?e???? ???e?H???'?????P?e~9????<)~?]?<I?e????????~? ??;?e??#???<)<h?e]???Q?QJx1?4#?QJx1???BQJx1???? ~????g~MQJ;??????<)?e]??I~M?mY?QJ[5];Y?<)<R ?e]???Q?QJx1?G??QJx1]??r??QJ?W;?e~M??#?<h]??? Q?QJx.???M?P?e??????~??e<??Y??x1?(??QJx1?????9 ?gQJ?W~???%?x1?????b?IQJx1?8~M???W????x. ???5???M????????#????C?????[??<??:[6]?e V.1.14No.3???-?W:Y??????5???M?????????e?e???????-~???Q3Yz?iQ3?? ??#?~?]?%?<R????~?Qa?e<?%7?????P~???~?Y??mY? <x??x1#??~???????a?K?4#????MY??U<???]?%?Q??? ???5<???#?x. ~??4???w 1?i???q_?j???o?W#T???????[???????r?????g??Q[???? ?+.?a?K<????H??<D??~?.1993,5(1):54 2??#??r??~M??.??T<)Y??j?b?P1991,342--347 3Q6Y+???P???c???PQ6?~???W.????????~??????e???{Q3?? ~??mY???<????W?????P2000,4:20-21 4NelsoEW.Theaminoacidaschemicaltemptationfurtrout andwimon.FeedManagemmt,1991,42(3):6<?10 5#??D??<???<?????????????Q?QJ<??x???e??.?????H ???P1989,9:3 6????~<?P#5?????P?,?]???W.???C?????[?????mY??-Q3~? Y??'???e???????-.?5<??????P2000,24(2):180-184 ANALYSISOFPROTEIN,AMINOACIDSANDSOMEELEMENTS INMANTELLUMOFFOURKINDSOFFRESHWATERMUSSEL JIANGYan,LIUKe-wu,LIUXiao-wen,MINLi-e,SHIAn-jing,QIUAn-dong,1-1Jing,SUNQi-zhi (GillegeofLifeSciencv,Sick-Unicrrsity,Chengdu610064,Chim AbstractThecrudeproteincontentsinmantellumoffourkindsoffreshwatermusselweredeterminedandthe aminoacids(AA)wereanalyzed.Atomicabsorptionandplasmaabsorptionspectroscopywereusedtodetermine thecontentsof12metalelementsinthefreshwatermussels.Theresultsshowedthattheproteincontentsin driedmusselare41.34%forHyriposiscumingiiHea,22.03%forAnodontawoodianapacificaHeude, 32.1496forCristariaplicataLeach,and7.78%forLawprotulaLeai.Allofthefourmusselscontain17kinds ofAA.Amongthe12analyzedelementsinthefreshwatermussels,thecontentsofCuarethehighest,thecon- tentsofFe,Mn,Mg,Ca,ZnandCrarelessthanthoseofCu,whereasthecontentsofNi,Co,Pb,andHgaretlte least.Theseresultsshowedthatthemantellumofallthefourtestingmusselsaregoodproteinresources Key word word文档格式规范word作业纸小票打印word模板word简历模板免费word简历 sfreshwatermussel;proteinofmantellum;aminoacid;elements ?P??i6i6~?~?~??P?P?'x1?P?'?P??<??Px1???P??x1~?x1??i6~?Y?x.?P<??Pd?P<????P<t?Px1??x1??x1~?i6??x1i6<?<D..~??P~??6??Y?<????P??~??.?P~?~??e.?Pi6]?~?Y????P??x1<+x1??~??x1~i6?? ?a?K????]???(~9???rQR????:????#?????)?? NaturalMedicineInforlnation(MedicalJournalWatch:AbstractandComments) Ann-UtcermedicationLinkedto Coba]XminDeficiency Mitchell5I-RoekwoodK.Theassociationbetweenanti-ul- cermedicationandinitiationofcobalaminreplacementinolder persons.JCldnEpid-iol54(5):531-534,2001. Among125subjectsusinganti-ulcermedication(histanune- 2blockersorproto-pumpinhibitors),49hadstartedwbalamin replacementatfollow-up.Theauthorsrecommendthatanti-ul- cermedicationsbecautiouslyprescribedforolderpatientsbe- causeoftheassociationbetweenuseofthesemedicationsand colmlamindeficiency. .NPNotes:Theriskofcobalamindeficiencyacumng duringtreutrnentwithanti-ulcerntedicationis~thana mereassociation.Medicineswreakhavocwithnutrientabsorp- tionandutilization,andvitaminB12isespeciallyvulnerable tosuchinterference.Nutrientsupplementationshould,atthe veryleast,Q?aroutinem??unctQ?othertherapies. VitaminsCandEMayEase SymptomsofRadiationProctitis KennedyM,BruningaK,etal.Successfulandsustained treatmentofchronicradiationpmctitiswithantioxidantvitamins EandC.An,JGrit-terol96(4):1080-'1084,2001. Patientswithradiationproctitiswereaskedtotakeboth 400IUofvitaminEand500mgofvitaminCthreetimesdaily Thevitaminsproducedbenefitsbyreducingdiarrheaandincon- tinence,andthebenefitscontinuedduringsupplementation#o?? year .NPNotes<?xO'rcctitisisacommonsideeffect~?raduc- tionthemQ?tothepelvicregion.Itoftenpreventspatientsfrom returningtotheirusuallifest??i6aftersurgery.Thisisasig- nificantstudy,whichclearlyshowsthatvitaminsupplements promotehealingandreducethesideeffectsofcancertherapy VitaminEMayDecreaseOxidativeStns Q?ChronicOtitisMediaandTonsillitis ShuklaGK,GuptaR,etal.RoleofvitaminEandsurgery inoxyfreeradicalsystemoferythrocytesincasesofchronicton- sillitisandchronicsuppurativeotitismedia.AsiaPacificJPhar- macol14(4):143-145,2000, Inthissmallstudy,vitaminEsupplementsdecreasedoxide tivestressamongpatientssufferingfromchronicotitismediaand chronictonsillitis .NPNotes:EarinfationsandtonsillitisiBx.bothin- flammatorydisorders,i??tencausedQ?foalallergiesmthe.,- thanin<?etions.VitaminEmightreduceinflammation??loa,- eringl}lsofCrmctimprotein,aproinflammatorysubstance