产黄芩苷内生真菌的筛选与鉴定

产黄芩苷内生真菌的筛选与鉴定 1001-6880 ( 2012 ) 06-0819-05 魏希颖1张黎光1,2姚玉秀1，尚菲1，马彩霞1刘清梅1 1 陕西师范大学生命科学学院，西安710062；2陕西省微生物研究所，西安710043 摘要：从药用植物黄芩根、茎、叶和花中分离得到17株内生真菌，其发酵液对10种指示菌进行抑菌活性测定， 并通过薄层色谱(TLC)和高效液相色谱(HPLC)对所分离到的内生真菌代谢产物进行分析。结果显示，所分离 到的内生真菌中12株至少对一种指示菌有抑菌活性，其中3株（G2、J4和J5）具有较广的抑菌作用。从黄芩茎 和花中分离得到的2株内生真菌J1、H3可以在人工培养基中产生黄芩活性成分—黄芩苷，结合菌落形态特 征、显微观察及ITS序列分析，初步鉴定这2株菌株均属于青霉菌属。 黄芩；植物内生真菌；抗菌；黄芩苷 Q939.9 A Selection and Identification of Baicalin-Producing Endophytic Fungi Isolated from Scutellaria baicalensis Georgi WEI Xi-yingZHANG Li-guangYAO Yu-xiuShang Fei MA Cai-xiaLIU Qing-mei 2011-04-13 2011-11-05 陕西师范大学2010重点教学改革研究项目资助课题 ( ZDXMZYJS065)；中央高校基本科研业务费资助项目 (GK201002008) 万方数据 物研究与开发 万方数据 真菌的筛选与鉴 万方数据 物研究与开 万方数据 @@1. Li HY（李海燕），Liu L（刘丽）.Recent advances on bioac tive compouds producing endophytes. Nat Prod Res Dev（天 然产物研究与开发）,2004,16:482-485. @@2. Stierh A,Strobel GA,Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Sci, 1993,260:214-216. @@3. Li HL(李海丽).Progress of Radix Scutellariae.Chem Eng Equip（化学工程与装备），2008，4:100-102. @@4. Schulz B, Sucker J, Atst HJ,et al. Biologically active second ary metabolites of endophytic Pezicula species. Mycol Res, 1995,99 : 1007-1011. @@5.Dong XC(董学畅)，Han Y(韩勇)，Yang HY(杨海英)，et al. Prepatation of Standard Baicalin and Determination by SPE-HPLC. Yunnan Chem Technol（云南化工），2005,32 (6)：28-31. @@6. Pei YP（佩云萍），Diao YH（刁雨辉），Wu ZH（吴正红）. TLC identification of Scutellaria baicalensis, golden cypress and Acacia catechu in Scabbed-over ding. Jiangsu Pharm Clin Re（江苏药学与临床研究）,2003,11(4)：28-29. @@7.China Pharmacopoeia Committee. ChPⅠ（中国药典Ⅰ部）. Beijing:Chemistry Industry Press,2005. 211-212. @@8. Gardes M,Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycor rhizae and rusts. Mol Ecol , 1993,2 : 113-118. @@9. Wei JC（魏景超）.Taxonomic Outline of The Manual of Sys tematic Mycetology（真菌鉴定手册）.Shanghai: Shanghai Scientific and Technical Publishers, 1979.501-508. @@10. Cui JL（崔晋龙）,Fan L(范黎),Ding C（丁翠）,et al. Screening of Anti-microbe Activity and Identificationof En dophytic Fungus in Cultivited Scutellaria baicalensis. Acta Bot Boreal-Occident Sin（西北植物学报），2007,27:1384- 1388. @@11. Bugni TS, Ireland CM. Marine-derived fungi: a chemically and biologically diverse group of microorganisms. Nat Prod Rep,2004,21 : 143-63. @@12. Zhang G(张根). An Endophytic Fungus from Camptotheca Acuminate that produce CPT and research of biology of CPT. Guiyang: Guizhou University, MSc. 2007. 万方数据 产黄芩苷内生真菌的筛选与鉴定 作者： 魏希颖， 张黎光， 姚玉秀， 尚菲， 马彩霞， 刘清梅， WEI Xi-ying， ZHANG Li-guang ， YAO Yu-xiu， Shang Fei， MA Cai-xia， LIU Qing-mei 作者单位： 魏希颖,姚玉秀,尚菲,马彩霞,刘清梅,WEI Xi-ying,YAO Yu-xiu,Shang Fei,MA Cai-xia,LIU Qing-mei(陕西师范大学生命科学学院,西安,710062)， 张黎光,ZHANG Li-guang(陕西师范 大学生命科学学院,西安710062;陕西省微生物研究所,西安710043) 刊名： 天然产物研究与开发 英文刊名： Natural Product Research and Development 年，卷(期)： 2012,24(6) 参考文献(12条) 1.李海丽 Progress of Radix Scutellariae 2008 2.Stierh A;Strobel GA;Stierle D Taxol and taxane production by Taxomyces andreanae,an endophytic fungus of Pacific yew 1993 3.张根 An Endophytic Fungus from Camptotheca Acuminate that produce CPT and research of biology of CPT 2007 4.Bugni TS;Ireland CM Marine-derived fungi:a chemically and biologically diverse group of microorganisms 2004 5.崔晋龙;范黎;丁翠 Screening of Anti-microbe Activity and Identificationof Endophytic Fungus in Cultivited Scutellaria baicalensis 2007 6.李海燕;刘丽 Recent advances on bioactive compouds producing endophytes 2004 7.魏景超 真菌鉴定手册 1979 8.Gardes M;Bruns TD ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts 1993 9.China Pharmacopoeia Committee 中国药典Ⅰ部 2005 10.佩云萍;刁雨辉;吴正红 TLC identification of Scutellaria baicalensis,golden cypress and Acacia catechu in Scabbed-over ding 2003(04) 11.董学畅;韩勇;杨海英 Prepatation of Standard Baicalin and Determination by SPE-HPLC 2005(06) 12.Schulz B;Sucker J;Atst HJ Biologically active secondary metabolites of endophytic Pezicula species 1995 本文链接：http://d.g.wanfangdata.com.cn/Periodical_trcwyjykf201206024.aspx