蓝桉叶油脚油气相色谱分析研究

蓝桉叶油脚油气相色谱分析研究 第17卷第2期 1997年6月 韩产化学与工业 ChemistryandIndustryofForestProducts VoL.17No.2 JuBe1997 GASCHROMATOGRAPHYOFRESIDUEFR0M FRACTIONALDISTILLATIONOFjE『CAyPrS GLOBULUSLEAFOIL’ ZhaoZ.1 ZhaoZhendongSunZhenLiangZhiqinWangYon (ResearchInstituteofChemicalProcessingandUtilizationofForest Products,CAFNanjing210042) AbstractMorethan70componentsintheresiduesofEucalyptusglobulusLeafoil weredetected,andamongthem48componentswereidentifiedwithGCandGC— MS.ThemaincomponentsWereidentifiedasa-terpineoi(SampleA27.9%tSample B5.5),aromadendeene(SampleA17.3tSampleB26.8),atloaromeden— drene,guaienes,gurjunenes,epiglobulo[,ledol.diepipalustrolandothers. KeywordsResiduejEucalyptusglobulusleafoiltComponent InSouthernChina,EucalyptusglobulusLabil1.iswidelyplantedasaquick?growing economicplant.Bytreatingleaveswithsteamdistillation,4t000tO6.000tonsofcrude E.globulusIearoiIcanbeobtainedannually.Fractiona1distilIationofthecrudeoiIsup— pliesfineeucalyptusoil(oneofthemainnaturalnavors),andresidue(about10)con— sistingofhigh—boilingpointcompounds.Thereis,however,noefficientutilizationforthe residue,becausejtscomponentsarenotquiteclear.Usuallyit_ssoldjnIowpriceoreven putaside.InYunnanProvinceabout300tonsofsuchresidueisexpectedtobeutilizedev. eryyear. A1otofreferencesonthecomponentsofE.globulusleafoilr,canbefoundinIiter- atures,butfewonthecomponentsofthedistilhdresidue.Nizharadzeet口Z.Ca]analyzed thecomponentsoftheresiduesofE.viminalisandE.dnerea,andreportedthatthemain componentwasselinenoI(about30)intheformer,anda-terpineoI(40,5O)inthe Iatter.FormoreefficientutilizationoftheresiduefromE.globulusleafoiIprovidedin YunnanProvince,adetailedinvestigationontheircomponentswithGCandGC.MSanaly. sishasbeencarrledOUt. 1Experimental 1.1Samples Pmj~tsupportedbyFoundationofScientificResearchandDevelopmentofChineseAcademyof Forestry Receivedon1996-O8一l5 林产化学与工业第17卷 SampleswerethefreshlyrecoveredfromtwoflavorplantsinKunminginFebruary 1995.BothweredarkocherousoilsandlabeledasSampleAandSampleB.respectively. 1.2Instrumental GaschromatographywascarriedOUtbyaChuanfenGC一7typeinstrumentwithSE一54 (0.36mm,L34m,8O,200C,4”C/rain)andOV一101(0.34mm,L30m,80, 200?,4C/rain)glasscapillarycolumnsandbyaShimadzuGC-7AinstrumentwithDB? 5quartzcapillarycolumn(0.3lmm,L30m,60,260?,4C/rain),bothinstruments withFID(Hydrogenflameionizationdetector)andnitrogencarriergas.GC—MSanalysis wascarriedOUtbyJMS-D3OOGC/MS/COMPtypemachineswithSE-54quartzcapillary column(0.25mm,L30nl,6O,255C,4?/rain)forGC;andwith70eVionization voltageandl90?ionizationsourcetemperatureforEI—MS. 2Resultsanddiscussion Fortheresidues,thebestGCseparationcanbeachievedbySE-54(0.25mm.L 30m)andDB-5quartzcapillarycolumns(0.31mm,L30m).Therearemorethan70 componentsineachresidueofwhichthetypicalgaschromatographiealchartisillustrated inFig.1forSampleA.Themaincomponents(asshowninTable1)wereidentifiedwith GC-MS[]anddeterminedquantitativelywithpeakareamethod. Fig—lTheGCchromatogramforresiduesampieA AsshowninTable1,themaincomponentofSampleAwasct-terpineo1withan amountof27.9.Thiscomponentmaybeanimportantresourceofnatural?-terpineol widelyutilizedintheperfumeryandflavors.Thesecondcomponentwasaromadendrene (17.3).Otherswerealloaromadendrene.guaienes,epiglobulo1,1edo1,gurjunenes, diepipalustro1.etc.Afterfractionaldistillationthesecomponentswereenrichedtoatota1 amountof33.2intheresidue(A),whichcanbeusedasprecursorsofbio-activeazo 第2期林产化学与工业 lenoidthroughdehydrationanddehydrogenation. Table1Thecon~ntsofthecomponentsintheSamplesAandB 40林产化学与工业第17卷 ThemaincomponentofSampleBwasaromadendrenewithanamountof26.8.The secondonedifferedfromSampleAwasterpinylacetatewhichisanaturaIresourceofper— fumeryandflavors.Thetotalprecursorsofazulenoidwereenrichedtoashighas49.2. AzuLenecompoundsfoundinEuca”ptusglobulusleafoil[1--g—couldnotbedetectedinthe residuesSampleAandSampleB,ofwhichthelowboilingpointcompoundssuchasl,8一 cineole,?一pineneetc.hadbeenrectifiedoutalready. 3Conclusion ThemaincomponentsoftheresiduesSamplesAandBwerearomadendrene(A: 17.3;B:26.8),d—terpineol(A:27.9,B:5.5),terpinylacetate(A:6.5;B: l6.4)andsomeprecursorycompoundsofazulenoid.TheresidueofEucalyptusglobulus leafoilwillheasuper—valuablyimportantresourceofazulenoidandnaturald—terpineol and/orterpinylacetate. Referenc 1BoetensHM.EssentmloilsandaromachemicalsfromEucalyptusglobutusLahi[1..Perfum.Flavor., 1984(Pub1.1985),9(6):1.3,6,8,10.12,13 2ValeJC,ProencadaCunhaA.AnalyticalstudyofessentialoilofEucalyptusglobulusfromtheeonti. nent.Bo1.Fac.,-”Farm..Univ.Coinhra.Ed.Cient,1972,32:238,26I,C.A.79:96829 3NizharadzeAN,BagaturiyaNSh,DemenyukMNetat.Studyofwastesfromtheproductionofhigh qualityEucalyptusoil.Maslo—Zhir.Prom—st.t1977.(7):31,32 4JapanPerfumeryandFlavoringAssociation.Spectralatlasofterpefmsandrelatedcompounds.Tokyo: HirokawaPublishingCo.?1973 5StenhagenE,AbrahamssonS,MclaffertyFM.Registryofmassspectradata,JohnWileyandSons, 1975 6MelaffertyFM,StaufferDB.TheWiley/NBSregistryofma3sspectraldata,JohnWileyandSons. 1989 7TheMassSpectrometryDataCentre.Eightpeakindexofillas$spectra.3rdEd.,TheRoyalSocietyof Chem[stry,1983 7]一o 蓝桉叶油脚油气相色谱分析研究 孙震粱志勤王艳18 (中国林业科学研究院林产化学工业研究所南京210042) 摘要对取自昆明两个香科厂的蓝枝叶油脚油进行系统的GC及GCMS分析,在已检出的70 亲个成 分中鉴定了48个化台物. 证明 住所证明下载场所使用证明下载诊断证明下载住所证明下载爱问住所证明下载爱问 其主要成分为挫油醇(样品A:27.9F样品B:5.5)和(+)一香术兰 烯 (A:17.8;B:268).其它成分有愈刨术烯,别香术兰烯,表蓝按醇,喇叭茶醇,古芸烯,双表喇叭茶醇 (Diepipah]stro1)等.它们可作为奠的前驱体化台物使用.即可经脱木,脱氢转化为奠类化台物. 这表明进行 脚油的鲧加工利用将会有较广 关键词脚油:蓝按叶油f组成