二氧化碳培养箱（Carbon dioxide incubator）

二氧化碳培养箱（Carbon dioxide incubator） 二氧化碳培养箱(Carbon dioxide incubator) Selection and use of CO2 incubator The research and production of CO2 incubator is widely used in medicine, immunology, genetics, microbiology, agricultural science and pharmacology, has become one of the most commonly used conventional instruments in the field of laboratory, through the simulation in culture box to form a similar cell / organization in vivo growth environment such as constant pH (pH value: 7.2-7.4), stable temperature (37 degrees C) relative humidity, the higher the level of CO2 (95%), stable (5%), the cell / device for tissue cultured in vitro. CO2 search dot com incubator is controlled to simulate 3 basic variables in vivo environment: the stability of the CO2 level, temperature and relative humidity. To have a stable training environment, we must consider these three factors, when purchasing, we should have a certain understanding of these "top priority" to choose their own instruments. However, other aspects of the "small" factors can not be ignored, because these will affect the use value and life of the instrument. When choosing, it should be considered from various factors. 1. temperature control: Maintaining constant temperature in the incubator is an important factor in maintaining cell growth. There are two types of heating structures available for carbon dioxide incubator: gas jacket heating and water jacket heating. Although the two heating systems are accurate and reliable, they all have their respective advantages and disadvantages. The water jacket incubator maintains a constant temperature through an enclosure heated by an independent hot water compartment. The hot water circulates in the tank through natural convection, and the heat is transferred to the inside of the box by radiation, thus keeping the temperature constant. The unique design of the water jacket type has its advantages: water is a good insulation material, when faced with failure, jacket type system can reliably keep the incubator temperature accuracy and stability (to maintain constant temperature time is 4-5 times the gas sleeve type system). If your experiment environment is not stable (such as electric limit, or frequent power outages and needs) conditions, in order to keep stable for a long time now, the water jacket design co2incubator is your best choice. The gas jacket heating system is used to heat the gas in the box directly through the heater in the box. The gas sleeve design can quickly restore the temperature stability in the case of the frequent change of the temperature caused by the frequent switching of the box door. Therefore, compared with the water jacket type, the gas sleeve type has the characteristics of fast heating and quick recovery of temperature, which is especially beneficial to the short term culture and the frequent switch of the box door. In addition, for the user, the air sleeve design is simpler than the water jacket type (water jacket type needs to add water, empty and clean the tank, and often monitor the operation of the water tank). In the purchase of gas type incubator, must pay attention: in order not to affect the cultivation of incubator should also have a fan to ensure the circulation of the air, rapid recovery of this device helps the temperature and relative humidity, CO2. In addition, some types of co2incubator also have the door and the auxiliary heating system, the heating system can provide a good cell door, humidity environment, guarantee to maintain the balance of osmotic pressure, and can effectively prevent the formation of condensation water to maintain the humidity and temperature of the incubator. If your culture environment needs precise control, then the auxiliary system is essential. 2.CO2 control: CO2 concentration detection can be measured by two control systems -- infrared sensor (IR) or heat conduction sensor (TC). When co2incubator the door is open, the CO2 from the box body leakage, the sensor can detect the decrease of the concentration of CO2, and make a timely response, re injection of CO2 to return to the original preset level. The principle of heat conduction sensor (TC) monitoring the concentration of CO2 is by measuring two electric heating regulators (one regulator is exposed to the box environment), The other is closed, and the resistance changes between them. The change of the CO2 concentration in the box will change the resistance between the two electric heating regulators, thus prompting the sensor to react to achieve the role of regulating the level of CO2. One disadvantage of TC control system is that the change of temperature and relative humidity in the box will affect the accuracy of the sensor. When the box door is frequently opened, not only the CO2 concentration, temperature and relative humidity will also fluctuate greatly, thus affecting the accuracy of the TC sensor. The control system is not applicable when precise culture conditions and frequent opening of the incubator door are required. Infrared sensor (IR), as another selectable control system, has more precise CO2 control capability than TC system. It detects CO2 level through an optical sensor. The IR system includes an infrared emitter and a sensor, when the box body CO2 absorbs some infrared transmitter, the sensor can detect infrared radiation to reduce the amount and the amount of infrared radiation is absorbed in the box body corresponds to the level of CO2 concentration, which can draw the box CO2. Because the IR system is not affected by changes in temperature and relative humidity, it is more accurate than TC systems, especially for cell cultures that require frequent opening of culture box doors. However, this system is more expensive than the TC system, which should be combined with the budget consideration. 3. relative humidity control: The control of relative humidity in the incubator is very important. Maintaining sufficient humidity level will ensure that the culture failure is not caused by excessive drying. Large co2incubator is used for steam generator or spray to control the relative humidity level, while most small and medium-sized incubator is the humidity control panel (humiditypans) the effect of moisture evaporation (the relative humidity level up to 95-98%). Some incubators have a moisture reservoir (humidityreservoir) that can retain moisture on the heated control panel, which enhances evaporation. The reservoir can increase relative humidity to 97-98%. However, the system is also more complex, because of the complexity of the structure increases, some unexpected problems will appear in the process of use. 4. micro processing control system: The instrument every user can hope for is easy to use, micro processing control system and other accessories (such as high temperature automatic control and alarm device, alarm device, CO2 password protection, automatic calibration system and so on) the use of the carbon dioxide cultivation box operation and control are very simple. The micro processing control system is an operating system that maintains the steady state of temperature, humidity and CO2 concentration in the incubator. For example, the PIC microprocessor control system, it can strictly control the concentration of gas and the loss down to a very low level, to ensure the training environment is constant, and can ensure the long-term training in the process of the temperature accurately, and a LED display, can be set, correction of temperature and concentration of CO2. Different micro processing system although the name is not the same, but the principle and the control effect is not very different, the purchase should not be too concerned about the difference between their names, the key is to feel easy to use, easy to operate, but also to be able to achieve the required control accuracy. In addition, I think an alarm system is indispensable, it allows you to know the incubator in time and respond to the situation, thereby minimizing the loss, to ensure continuity of the experiment. Some of the incubator have sound / light alarm device, when the temperature changes to + 0.5 degrees, or CO2 concentration changes to + 5%, it will automatically alarm; some have CO2 concentration abnormal alarm display function. These devices are designed for the convenience of users to reduce tedious and tedious experimental process. Control of 5. pollutants: Pollution is a major factor in the failure of cell culture, so manufacturers of carbon dioxide incubators have designed many different devices to reduce and prevent pollution, Cultivation of low concentration gas explosion and low gas ignition items and toxic substances. 11. correctly use and pay attention to the maintenance of the instrument, so that it is in good working condition, can extend the service life of the instrument; 12. after the refrigeration system stops working, wipe the working chamber and the glass observation window with soft cloth; 13. instruments in the continuous working period, every three months should be a regular check; check whether there is water, dirt falling into the refrigerating element and exposed motor; cleaning the compressor, the condenser dust and dirt; check the fuse, control components and fastening screws; 14. long term use of the instrument, natural wear is a normal phenomenon, should contact the manufacturer for repair.