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「光合成細菌―研究材料としての魅力―」(可编辑)「光合成細菌―研究材料としての魅力―」(可编辑) 「光合成細菌―研究材料としての魅力―」 NEWS LETTER Vol 20 NO 2 August 2010 THE JAPANESE SOCIETY OF PHOTOSYNTHESIS RESEARCH 60 ―― 65 CO2 72 ―― 77 78 79 84 93 100 109 1 118 1 120 3 121 2 122 2 123 70 124 124 125 126 128 129 ...

「光合成細菌―研究材料としての魅力―」(可编辑)
「光合成細菌―研究材料としての魅力―」(可编辑) 「光合成細菌―研究材料としての魅力―」 NEWS LETTER Vol 20 NO 2 August 2010 THE JAPANESE SOCIETY OF PHOTOSYNTHESIS RESEARCH 60 ―― 65 CO2 72 ―― 77 78 79 84 93 100 109 1 118 1 120 3 121 2 122 2 123 70 124 124 125 126 128 129 129 130 20 2 2010 TOPICS 1 4 LC-MSMS NDH LC-MSMS1 N D H 2 11 ndhA-ndhK 2 1997 C NDH 4 LC-MSMS 19992003 N D H N NDHC 4 1 C NDH 4 1 NAD-ME NDH NAD-MEC 4 Blue Native PAGE BN- 12kg PAGE Tips 2 NAD P H Dehydrogenase NDH E-mail takabayashipoplowtemhokudaiacjp 60 20 2 2010 n-Dodecyl-beta-D-maltoside DM NDH II com投稿赚 钱 T - Blue-Native PAGE nitroblue DNANDH tetrazolium chloride NBT N D H SDS-PAGE N D H N NDF1 NDF2 NDF4 NDF5 NDF6 PPL2 3-6 LC-MSMSC4 NADP-ME T- D N A N D H 2 20032006 L C - 3 M S M S NLC-MSMS N D H 1 2 2 LC-MSMS N D H N D H MS D N A EST 3 BN-PAGETips ATTED- 61 20 2 2010 Buffer buffer pH 4?pH 70 bufferbufferBis-Tris 8 Imidazole Cathode buffer CBB buffer CBB 2 045 μm 1BN-PAGE CBB SN-2090 Str Thy ATTO AE-6530M Str RubisCO 8Thy I I P S I I SC II PSII-D I-LHCI PSI-LHCI LHCII 3LHCII- 2A B T G l y c e r o l BN-PAGE Blue Native PAGE BN-PAGE Native CBB G-250 Native PAGE 10 Tips BN-PAGETips BN-PAGE Schgger 7 Nature Protocols 2BN-PAGE 8 A B N - PAGE B 62 20 2 2010 2 A 1 mA 1312 BN-PAGE MS CBB-freeCathode buffer CBB BN-PAGE 50 glycerol 50 glycerol 8 0 ? SDS-PAGE C B B MS DM 10μg40μg 4μg8μg chl C B B 2 wv 5 LC-MSMSSDS-PAGE BN-PAGE CBB C B B 4 ? CBB 4 LC-MSMS - BN-PAGE -80?1 D M N D H overnight 50V 10 overnight 63 20 2 2010 Received July 7 2010 Accepted July 22 2010 Published Endo T and Sato F 2008 NDF6 a thylakoid protein specic to terrestrial plants is essential for activity of August 31 2010 chloroplastic NAD P H dehydrogenase in Arabidopsis Plant Cell Physiol 49 1066-1073 5 Takabayashi A Ishikawa N Obayashi T Ishida S Obokata J Endo T and Sato F 2009 Three novel subunits of Arabidopsis chloroplastic NAD P H 1 Takabayashi A Kishine M Asada K Endo T and dehydrogenase identied by bioinformatic and reverse Sato F 2005 Differential use of two cyclic electron genetic approaches Plant J 57 207-219 ows around photosystem I for driving CO - 2 6 Ishida S Takabayashi A Ishikawa N Hano Y concentration mechanism in C4 photosynthesis Proc Endo T and Sato F 2009 A novel nuclear-encoded Natl Acad Sci USA 102 16898-16903 protein NDH-dependent cyclic electron ow 5 is 2 Majeran W Zybailov B Ytterberg AJ Dunsmore essential for the accumulation of chloroplast NAD P H J Sun Q and van Wijk KJ 2008 Consequences of dehydrogenase complexes Plant Cell Physiol 50 C differentiation for chloroplast membrane proteomes 4 383-393 in maize mesophyll and bundle sheath cells Mol Cell 7 Schgger H and von Jagow G 1991 Blue native Proteomics 7 1609-1638 electrophoresis for isolation of membrane protein 3 Ishihara S Takabayashi A Ido K Endo T Ifuku complexes in enzymatically active form Anal K and Sato F 2007 Distinct functions for the two Biochem 199 223-231 PsbP-like proteins PPL1 and PPL2 in the chloroplast 8 Wittig I Braun HP and Schgger H 2006 Blue thylakoid lumen of Arabidopsis Plant Physiol 145 native PAGE Nat Protoc 1 418-428 668-679 4 Ishikawa N Takabayashi A Ishida S Hano Y The Strategies for Analyzing Protein Complexes Suitable for Genome Generation Atsushi Takabayashi The Institute of Low Temperature Science Hokkaido University 64 20 2 2010 TOPICS 1 day-1 CO 2 1520 gDW m-2 day-1 9 0 20cm20 gDW m-2 day-1 01 gDW g 3 20 gDW m-2 day-1 2 174 kJg 174 kJ 19801990 2 -2 1 1 m 50 g 50 gDW m E-mail omataagrnagoya-uacjp 65 20 2 2010 N 2 40 kJgN 1 2 1 kg 10 cm 3000 rpm 10174 kJ 049 kJ 04 kJ 2 4 1 100? 4 100 kcal418 kJ 4 5 2 p H 4 12 2 13 3 1N2 N 2 66 20 2 2010 5-1 5-7 89 10 5-3 N 2 3 6 7 Spirulina Arthrospira Dunaliella s a l i n a p H 5-3 Pseudochoricystis ellipsoidea CN 6 1112 2040 5-2 Synechococcus elongatus Synechocystis sp PCC 6803 CN56CN 13 7 CN 5 15 14 15 16 3 CN 15 C 5 0 2 200910 17 54 072 Spirulina Arthrospira platensis kgC 75 kgC 46 30 4 18 kg 65 kgN 67 20 2 2010 1kgC75 87 1 2 21-23 24 812 18 kg 1 kg 235 3 0 CO2 5-5 N 2 N O x 1 9 NO CO x 2 20 6 2 5-4 68 20 2 2010 Azolla Anabaena azollae 2526 8 ― ― 7 Synechocystis sp PCC6803 10 ACP A C P CoA S 5 1 013 g 10 2 Pseudochoricystis ellipsoidea 7 42 2 7 Synechococcus elongatus PCC7942 9 2 8 69 20 2 2010 cyanobacterium Arthrospira Spirulina platensis NIES-39 DNA Res 17 85–103 5 Hemschemeier A Melis A and Happe T 2009 Analytical approaches to photobiological hydrogen production in unicellular green algae Photosynth Res 102 523-540 6 2006 6 46-52 7 Sakurai H and Masukawa H 2007 Promoting R D in photobiological hydrogen production utilizing mariculture-raised cyanobacteria Mar Biotechnol 9 128-145 9 8 Sakai M Ogawa T Matsuoka M and Fukuda H 1997 Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium Synechococcus sp PCC 7942 which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae J Ferment Bioeng 84 434-443 9 Takahama K Matsuoka M Nagahama K and Ogawa T 2003 Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus J Biosci Bioeng 95 302-305 10 Liu X Brune D Vermaas W and Curtiss R III 2010 Production and secretion of fatty acids in genetically engineered cyanobacteria Proc Natl Acad Sci USA published online before print doi 101073 pnas1001946107 11 Anderson J W and Beardall J 1991 Molecular Activities of Plant Cells Blackwell Scientic Received July 12 2010 Accepted August 6 2010 Publications Oxford UK Published August 31 2010 12 2006 4 com投稿赚钱 maffgojpsehikijun02touhoku0207fukusima 020601sehikijun020601contenntshtml 13 Redeld AC Ketchum BH and Richards FA 1 Qiu Q and Hlavacek V 2010 Energy estimation on 1963 The inuence of organisms on the composition CRN process of y ash as a slow-release nitrogen of sea-water in the Sea 2nd edition Hill N Ed pp fertilizer Ind Eng Chem Res 49 5939–5944 26-77 Wiley New York USA 2 Maurer M Schwegler P and Larsen T A 2003 14 Langner U Jakob T Stehfest K and Wilhelm C Nutrients in urine energetic aspects of removal and 2009 An energy balance from absorbed photons to recovery Water Sci Technol 48 37-46 new biomass for Chlamydomonas reinhardtii and 3 von Blottnitz H and Curran M A 2007 A review of Chlamydomonas acidophila under neutral and assessments conducted on bio-ethanol as a extremely acidic growth conditions Plant Cell Environ 32 250–258 transportation fuel from a net energy greenhouse gas 15 Yoshida T Hairston NG and Ellner SP 2004 and environmental life cycle perspective J Clean Prod 15 607-619 Evolutionary trade-off between defence against grazing 4 Fujisawa T Narikawa R Okamoto S Ehira S and competitive ability in a simple unicellular alga Yoshimura H Suzuki I Masuda T Mochimaru M Chlorella vulgaris Proc R Soc B 271 1947-1953 16 Takaichi S Awai K Sek
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