首页 Port-a-Patch数据及应用

Port-a-Patch数据及应用

举报
开通vip

Port-a-Patch数据及应用 Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn Port-a-Patch Data and Applications hERG通道: 德国 Nanion全自动膜片钳在 hERG通道上的应用: Stick...

Port-a-Patch数据及应用
Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn Port-a-Patch Data and Applications hERG通道: 德国 Nanion全自动膜片钳在 hERG通道上的应用: Sticky compounds, such as some hERG blockers, exhibit expected IC50 values with the Port-a-Patch®. The concentrations of the half maximal block were: 1.27 nM (astemizole), 8.9 nM (cisapride), 163.7 nM (flunarizine) and 11.0 nM (terfenadine). Cells were kindly provided by Cytomyx/Millipore, UK. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn 德国 Nanion全自动膜片钳应用:Quinidine对 hERG通道的抑制作用 A full dose response curve of quinidine acting on the hERG channel was obtained at physiological temperature (35 °C). The IC50 for quinidine at physiological temperature was 1.3 ± 0.2 μM (n = 5), similar to that obtained at room temperature (1.0 ± 0.03 μM, n = 3). Cells were kindly provided by Cytomyx/Millipore, UK. 德国 Nanion全自动膜片钳应用:温度对 hERG通道的影响 The image shows example traces for hERG mediated currents at 25 ± 2 °C (black) and 35 ± 2 °C (blue). The peak current amplitude was increased at 35 °C, and the rise time and decay time constants were faster at physiological temperature compared with those obtained at room temperature. Cells were kindly provided by Cytomyx/Millipore, UK. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn hEAG通道: 德国 Nanion全自动膜片钳应用:hEAG (hKv10.1) Block of hEAG whole cell currents after the internal application of 200 µM TEA. Data were kindly provided by Walter Stühmer, MPI for exp. Med., Göttingen. 德国 Nanion全自动膜片钳应用:P2X3通道 Shown are raw current traces from 1321 N1 cells expressing P2X3 receptors demonstrating the reproducibility of the whole cell responses. Currents were activated by 100 ms application of 10 µM ATP. The waiting time between sweeps was 120 s (holding −60 mV). Cells were kindly supplied by Evotec AG, Hamburg, Germany Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn GABA通道: 德国 Nanion全自动膜片钳应用:GABA通道应用 Ligand dependent activation of GABAA receptors can be recorded with the Laminar Flow Chamber with approx. 100 ms time constant for 10 μM GABA. Concentration dependent activation and desensitization of GABAA (α1β2γ2) receptors was obtained by applying 1, 3 and 10 μM GABA for 2.5 s at a time interval of 20 s. 德国 Nanion全自动膜片钳应用:在甘氨酸通道上的应用 The Laminar Flow Chamber can also be used for studying glycine receptors. The pharmacology of the hGlyRα1, expressed in a mouse fibroblast cell line (L-tk), was investigated. The EC50 for glycine was determined as 89 ± 2.7 μM (n = 10) which is in accordance with the literature. Cells were kindly provided by AstraZeneca, Sweden. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn 德国 Nanion全自动膜片钳应用:Na通道 rNav1.8 Shown are raw current traces (left) and averade data (right) of the current voltage (upper) and lidocaine dose response (lower) relationship of rNav1.8 stably expressed in ND7-23 cells. Cells were kindly provided by Cytomyx Millipore, UK. Shaker通道: 德国 Nanion全自动膜片钳应用:Shaker I Here we show recordings from HEK293 cells transiently expressing a Shaker K mutant that has its inactivation removed (Shaker-IR). The holding potential was -80 mV. The current-voltage relationship of the tail currents reveals a half-activating potential of -27 mV. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn Data were kindly provided by Dr. Kenton Swartz, NIH, Bethesda, USA. 德国 Nanion全自动膜片钳应用:Shaker II Block of Shaker-IR by Agitoxin2. Shown are current recordings from HEK293 transiently expressing Shaker-IR. Currents were elicited by a 500 ms step to +50 mV from a holding potential of -80 mV. K+ gradients are the same as for the IV curve experiment shown above. 200 nM Agitoxin2 almost fully blocks the Shaker K+ current. Data were kindly provided by Dr. Kenton Swartz, NIH, and Bethesda, USA. 德国 Nanion全自动膜片钳应用:超声穿孔通道(Sonoporation ) Current response of a non-transfected Chinese hamster ovary (CHO) cell to a train of ultrasound pulses (US) in the absence (A) and presence (B) of contrast agent. The holding potential was -80 mV. Ultrasound signals had a centre frequency of 5 MHz. Pulses of 50 cycles were repeated for 2 s every 10 ms. Data were kindly provided by Dr. Cheri Deng, University of Michigan, USA. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn TRP通道: 德国 Nanion全自动膜片钳应用:TRPM7 Shown are mTRPM7 (in HEK293 cell) raw current responses to a voltage ramp voltage protocol (−100 mV to 100 mV over 200 ms) recorded under control conditions and after subsequent internal perfusion with Mg2+ (left). Initially, currents showed some run-up before they became stable. The arrows in the time course on the right mark the time points for which the raw current traces are shown. 德国 Nanion全自动膜片钳应用:TRPM8 (Activated by Menthol) Menthol activated the TRPM8 channels expressed in HEK293 cells. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn 德国 Nanion全自动膜片钳应用:TRPM8/TRPV1 TRP channels recorded in HEK293 cells. Menthol (dark blue) activated the TRPM8 channels (shown on the left) and could be inhibited by genistein (blue). TRPV1 channels expressed in HEK293 cells (right) were stimulated by application of capsaicin. TRPV1 data were kindly supplied by Dr. David Cohen, Oregon Health and Service University, Portland, OR, USA. 德国 Nanion全自动膜片钳应用:TRPV1 Whole cell current responses from HEK 293 cells transiently expressing TRPV1 to a ramp voltage protocol (-100 mV to +100 mV). Capsaicin at a concentration of 2 μM reversibly activated the channel. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn Data were kindly provided by David Cohen, Oregon Health & Science University, Portland, USA. 德国 Nanion全自动膜片钳应用:TRPV1热敏感通道 ( Heat Activated Channels ) Raw traces of TRPV1 current responses to voltage ramps from –100 mV up to +100 mV. TRPV1 current at RT (28°C), during two stimulations by application of heated solution (34°C and 36°C), and after cooling down to RT (29°C). The picture on the right-hand side shows the current amplitudes as recorded at -100 mV and +100 mV plotted against time. Ca通道: 德国 Nanion全自动膜片钳应用:Cav2.2 Current-to-Voltage Relationship Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn Representative current responses of an individual HEK-293 cell expressing Cav 2.2 to a standard voltage protocol. Average current-voltage relationship (n = 10). The error bars reflect the standard error of the mean (S.E.M.). Cells were kindly provided by Millipore. 德国 Nanion全自动膜片钳应用:Cav3.2 –通道阻断剂(Channel Blockers) Shown are raw current traces (top) and average dose response curves (bottom) of CaV3.2 (T-type Ca2+- Channel) block by compounds as indicated. CaV3.2 is stably expressed in HEK293 cells. IC50s were 863 nM (Mibefradil), 27 μM (Nifedipine) and 52 μM Amiloride. Cells were kindly provided by Cytomyx Millipore, UK. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn 德国 Nanion全自动膜片钳应用:Cav3.2 - T-Type Ca Channels Shown are raw current traces (top left) and average peak current data (top right) of the current voltage relationship of CaV3.2 (T-type Ca2+- Channel) stably expressed in HEK293 cells. Activation and Inactivation plots were constructed (bottom). Half-activation and half-inactivating potentials were determined as -32 mV and -65 mV, respectively. Cells were kindly provided by Cytomyx Millipore, UK. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn 德国 Nanion全自动膜片钳应用:脂双层的重组通道(Bilayer Recordings of KcsA) The image shows a representative current trace recorded from a planar lipid bilayer in which purified KcsA channels were reconstituted (100 mM KCl, asymmetric pH; pH 4/pH 7). A ramp from -100 mV to +100 mV was used to evoke the single channel events. 德国 Nanion全自动膜片钳脂双层 记录 混凝土 养护记录下载土方回填监理旁站记录免费下载集备记录下载集备记录下载集备记录下载 :Bilayer Recordings of OmpF Channels were activated in the presence of spermine (0.5 mM). The downward spikes represent the closure events due to the effect of spermine. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn Data were kindly supplied by Prof. Mathias Winterhalter, Jacobs University, Bremen, Germany. 德国 Nanion全自动膜片钳应用:DNA Translocation in Hemolysin Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch®. Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany. 德国 Nanion全自动膜片钳应用:重组受体 Reconstitution of IP3 Receptors Representative traces of the IP3 (inositol-triphosphate) receptor were recorded at different voltages in the presence of IP3 (0.2 mM free Ca2+ and 1 mM Na2ATP, 140 mM KCl). Addition of 2 μM IP3 on the cytosolic side evoked openings of the IP3 receptor. Nanion Technologies Beijing Co., Ltd. Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn Data were kindly supplied by Prof. Colin Taylor, University of Cambridge, Cambridge, UK. 德国 Nanion全自动膜片钳应用:TRPM8 Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch®. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV. Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
本文档为【Port-a-Patch数据及应用】,请使用软件OFFICE或WPS软件打开。作品中的文字与图均可以修改和编辑, 图片更改请在作品中右键图片并更换,文字修改请直接点击文字进行修改,也可以新增和删除文档中的内容。
该文档来自用户分享,如有侵权行为请发邮件ishare@vip.sina.com联系网站客服,我们会及时删除。
[版权声明] 本站所有资料为用户分享产生,若发现您的权利被侵害,请联系客服邮件isharekefu@iask.cn,我们尽快处理。
本作品所展示的图片、画像、字体、音乐的版权可能需版权方额外授权,请谨慎使用。
网站提供的党政主题相关内容(国旗、国徽、党徽..)目的在于配合国家政策宣传,仅限个人学习分享使用,禁止用于任何广告和商用目的。
下载需要: 免费 已有0 人下载
最新资料
资料动态
专题动态
is_816792
暂无简介~
格式:pdf
大小:820KB
软件:PDF阅读器
页数:0
分类:
上传时间:2012-02-28
浏览量:29