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Port-a-Patch
Data and Applications
hERG通道:
德国 Nanion全自动膜片钳在 hERG通道上的应用:
Sticky compounds, such as some hERG blockers, exhibit expected IC50 values with the
Port-a-Patch®. The concentrations of the half maximal block were: 1.27 nM (astemizole),
8.9 nM (cisapride), 163.7 nM (flunarizine) and 11.0 nM (terfenadine).
Cells were kindly provided by Cytomyx/Millipore, UK.
Nanion Technologies Beijing Co., Ltd.
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德国 Nanion全自动膜片钳应用:Quinidine对 hERG通道的抑制作用
A full dose response curve of quinidine acting on the hERG channel was obtained at
physiological temperature (35 °C). The IC50 for quinidine at physiological temperature was
1.3 ± 0.2 μM (n = 5), similar to that obtained at room temperature (1.0 ± 0.03 μM, n = 3).
Cells were kindly provided by Cytomyx/Millipore, UK.
德国 Nanion全自动膜片钳应用:温度对 hERG通道的影响
The image shows example traces for hERG mediated currents at 25 ± 2 °C (black) and 35
± 2 °C (blue). The peak current amplitude was increased at 35 °C, and the rise time and
decay time constants were faster at physiological temperature compared with those
obtained at room temperature. Cells were kindly provided by Cytomyx/Millipore, UK.
Nanion Technologies Beijing Co., Ltd.
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hEAG通道:
德国 Nanion全自动膜片钳应用:hEAG (hKv10.1)
Block of hEAG whole cell currents after the internal application of 200 µM TEA.
Data were kindly provided by Walter Stühmer, MPI for exp. Med., Göttingen.
德国 Nanion全自动膜片钳应用:P2X3通道
Shown are raw current traces from 1321 N1 cells expressing P2X3 receptors demonstrating
the reproducibility of the whole cell responses. Currents were activated by 100 ms
application of 10 µM ATP. The waiting time between sweeps was 120 s (holding −60 mV).
Cells were kindly supplied by Evotec AG, Hamburg, Germany
Nanion Technologies Beijing Co., Ltd.
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GABA通道:
德国 Nanion全自动膜片钳应用:GABA通道应用
Ligand dependent activation of GABAA receptors can be recorded with the Laminar
Flow Chamber with approx. 100 ms time constant for 10 μM GABA. Concentration
dependent activation and desensitization of GABAA (α1β2γ2) receptors was obtained by
applying 1, 3 and 10 μM GABA for 2.5 s at a time interval of 20 s.
德国 Nanion全自动膜片钳应用:在甘氨酸通道上的应用
The Laminar Flow Chamber can also be used for studying glycine receptors. The
pharmacology of the hGlyRα1, expressed in a mouse fibroblast cell line (L-tk), was
investigated. The EC50 for glycine was determined as 89 ± 2.7 μM (n = 10) which is in
accordance with the literature.
Cells were kindly provided by AstraZeneca, Sweden.
Nanion Technologies Beijing Co., Ltd.
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德国 Nanion全自动膜片钳应用:Na通道
rNav1.8
Shown are raw current traces (left) and averade data (right) of the current voltage
(upper) and lidocaine dose response (lower) relationship of rNav1.8 stably expressed in
ND7-23 cells.
Cells were kindly provided by Cytomyx Millipore, UK.
Shaker通道:
德国 Nanion全自动膜片钳应用:Shaker I
Here we show recordings from HEK293 cells transiently expressing a Shaker K mutant that
has its inactivation removed (Shaker-IR). The holding potential was -80 mV. The
current-voltage relationship of the tail currents reveals a half-activating potential of
-27 mV.
Nanion Technologies Beijing Co., Ltd.
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Data were kindly provided by Dr. Kenton Swartz, NIH, Bethesda, USA.
德国 Nanion全自动膜片钳应用:Shaker II
Block of Shaker-IR by Agitoxin2. Shown are current recordings from HEK293 transiently
expressing Shaker-IR. Currents were elicited by a 500 ms step to +50 mV from a holding
potential of -80 mV. K+ gradients are the same as for the IV curve experiment shown
above. 200 nM Agitoxin2 almost fully blocks the Shaker K+ current. Data were kindly
provided by Dr. Kenton Swartz, NIH, and Bethesda, USA.
德国 Nanion全自动膜片钳应用:超声穿孔通道(Sonoporation )
Current response of a non-transfected Chinese hamster ovary (CHO) cell to a train of
ultrasound pulses (US) in the absence (A) and presence (B) of contrast agent. The holding
potential was -80 mV. Ultrasound signals had a centre frequency of 5 MHz. Pulses of 50
cycles were repeated for 2 s every 10 ms. Data were kindly provided by Dr. Cheri Deng,
University of Michigan, USA.
Nanion Technologies Beijing Co., Ltd.
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TRP通道:
德国 Nanion全自动膜片钳应用:TRPM7
Shown are mTRPM7 (in HEK293 cell) raw current responses to a voltage ramp voltage
protocol (−100 mV to 100 mV over 200 ms) recorded under control conditions and after
subsequent internal perfusion with Mg2+ (left). Initially, currents showed some run-up
before they became stable. The arrows in the time course on the right mark the time
points for which the raw current traces are shown.
德国 Nanion全自动膜片钳应用:TRPM8 (Activated by Menthol)
Menthol activated the TRPM8 channels expressed in HEK293 cells.
Nanion Technologies Beijing Co., Ltd.
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德国 Nanion全自动膜片钳应用:TRPM8/TRPV1
TRP channels recorded in HEK293 cells. Menthol (dark blue) activated the TRPM8 channels
(shown on the left) and could be inhibited by genistein (blue). TRPV1 channels expressed
in HEK293 cells (right) were stimulated by application of capsaicin.
TRPV1 data were kindly supplied by Dr. David Cohen, Oregon Health and Service
University, Portland, OR, USA.
德国 Nanion全自动膜片钳应用:TRPV1
Whole cell current responses from HEK 293 cells transiently expressing TRPV1 to a ramp
voltage protocol (-100 mV to +100 mV). Capsaicin at a concentration of 2 μM reversibly
activated the channel.
Nanion Technologies Beijing Co., Ltd.
Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn
Data were kindly provided by David Cohen, Oregon Health & Science University, Portland,
USA.
德国 Nanion全自动膜片钳应用:TRPV1热敏感通道 ( Heat Activated Channels )
Raw traces of TRPV1 current responses to voltage ramps from –100 mV up to +100 mV.
TRPV1 current at RT (28°C), during two stimulations by application of heated solution (34°C
and 36°C), and after cooling down to RT (29°C). The picture on the right-hand side shows
the current amplitudes as recorded at -100 mV and +100 mV plotted against time.
Ca通道:
德国 Nanion全自动膜片钳应用:Cav2.2 Current-to-Voltage Relationship
Nanion Technologies Beijing Co., Ltd.
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Representative current responses of an individual HEK-293 cell expressing Cav 2.2 to a
standard voltage protocol. Average current-voltage relationship (n = 10). The error bars
reflect the standard error of the mean (S.E.M.).
Cells were kindly provided by Millipore.
德国 Nanion全自动膜片钳应用:Cav3.2 –通道阻断剂(Channel Blockers)
Shown are raw current traces (top) and average dose response curves (bottom) of
CaV3.2 (T-type Ca2+- Channel) block by compounds as indicated. CaV3.2 is stably
expressed in HEK293 cells. IC50s were 863 nM (Mibefradil), 27 μM (Nifedipine) and 52 μM
Amiloride.
Cells were kindly provided by Cytomyx Millipore, UK.
Nanion Technologies Beijing Co., Ltd.
Tel:+86 10 82176388; email:info@nanion.cn; web: www.nanion.cn
德国 Nanion全自动膜片钳应用:Cav3.2 - T-Type Ca Channels
Shown are raw current traces (top left) and average peak current data (top right) of the
current voltage relationship of CaV3.2 (T-type Ca2+- Channel) stably expressed in HEK293
cells. Activation and Inactivation plots were constructed (bottom). Half-activation and
half-inactivating potentials were determined as -32 mV and -65 mV, respectively.
Cells were kindly provided by Cytomyx Millipore, UK.
Nanion Technologies Beijing Co., Ltd.
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德国 Nanion全自动膜片钳应用:脂双层的重组通道(Bilayer Recordings of KcsA)
The image shows a representative current trace recorded from a planar lipid bilayer in
which purified KcsA channels were reconstituted (100 mM KCl, asymmetric pH; pH 4/pH 7).
A ramp from -100 mV to +100 mV was used to evoke the single channel events.
德国 Nanion全自动膜片钳脂双层
记录
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:Bilayer Recordings of OmpF
Channels were activated in the presence of spermine (0.5 mM). The downward spikes
represent the closure events due to the effect of spermine.
Nanion Technologies Beijing Co., Ltd.
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Data were kindly supplied by Prof. Mathias Winterhalter, Jacobs University, Bremen,
Germany.
德国 Nanion全自动膜片钳应用:DNA Translocation in Hemolysin
Reconstituted Alpha hemolysin channels are constantly open at positive and negative
membrane potentials. Gating is observed as a result of the passage of a single stranded
DNA molecule through the pore. Recordings were performed on the Port-a-Patch®.
Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich,
Germany.
德国 Nanion全自动膜片钳应用:重组受体 Reconstitution of IP3 Receptors
Representative traces of the IP3 (inositol-triphosphate) receptor were recorded at
different voltages in the presence of IP3 (0.2 mM free Ca2+ and 1 mM Na2ATP, 140 mM KCl).
Addition of 2 μM IP3 on the cytosolic side evoked openings of the IP3 receptor.
Nanion Technologies Beijing Co., Ltd.
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Data were kindly supplied by Prof. Colin Taylor, University of Cambridge, Cambridge, UK.
德国 Nanion全自动膜片钳应用:TRPM8
Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The
recordings were made with the with the Port-a-Patch®. The channel was activated by
PIP2 and methanol. Currents were recorded at 100 mV.
Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical
School, Newark, NJ 07103, USA